陆地棉因其环境适应性广、产量高、产量潜力大而被广泛栽培。盐渍土抑制出苗期和苗期，进而影响陆地棉的生产。在这项研究中，从一个盐敏感品种（中 G5）和一个耐盐品种（中 07）开发了一个重组自交系（RIL）群体。使用 GBS 序列技术，构建了具有 1,154 个 bin 标记的遗传连锁图，其中包括 4,077 个单核苷酸多态性 (SNP)，覆盖了 1796.067 cM 的基因组长度。对2017—2019年盐胁迫和正常条件下控制田间出苗率、苗龄、产量和纤维品质性状进行QTL定位，探索陆地棉盐胁迫的遗传基础。共鉴定出 162 个幼苗 QTL，田间条件下 RIL 种群的产量和纤维品质性状。在产量相关的 5 个稳定 QTL 中，qBNP-ChrA13-1，QBW-ChrD12-1，QBW-ChrD12-2，QLP-ChrA01-1和QLP-ChrD05-1，分别在不同年份不同条件下确定的。两年内在多种条件下在纤维品质性状中检测到三个稳定位点qFL-ChrD11-3、qFM-ChrA13-2和qFM-ChrD05-1。这些结果对于了解棉花耐盐机制的遗传基础，促进棉花耐盐育种具有重要意义。

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Gossypium hirsutum is extensively cultivated due to its wide adaptability to environment, high production and better yield potential. The saline soil inhibited the emergence and seedling stage, and then effect on the production of upland cotton. In this study, a recombinant inbred lines (RIL) population was developed from a salt-sensitive variety (Zhong G5) and a salt-tolerance variety (Zhong 07). Using GBS-sequence technology, a genetic linkage map was constructed with 1,154 bin markers, which included 4,077 single nucleotide polymorphisms (SNPs) covering the 1796.067 cM of genome length. We performed QTL mapping controlling field emergence rate, seedling age, yield and fiber quality traits under salt stress and normal conditions in 2017—2019 years, to explore genetic basis of salt stress in upland cotton. There were 162 QTLs identified for seedling, yield and fiber quality traits from RIL population in field conditions. In the yield-related, five stable QTLs, qBNP-ChrA13-1, qBW-ChrD12-1, qBW-ChrD12-2, qLP-ChrA01-1 and qLP-ChrD05-1, were identified under different conditions in different years. Three stable loci, qFL-ChrD11-3, qFM-ChrA13-2 and qFM-ChrD05-1, were detected in fiber quality traits under multiple conditions in two years. These results could be useful in understanding the genetic basic of the salt tolerance mechanisms of cotton, and facilitating cotton salt tolerance breeding.