甘蓝型油菜不同发育时期株高QTL的动态分析 |
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DOI: 10.3724/SP.J.1006.2012.01802 甘蓝型油菜不同发育时期株高 QTL 的动态分析
谢田田 1,2
陈玉波 2,3
黄吉祥 2
张尧锋 2
徐爱遐 4
陈
飞 2
倪西源 2
赵坚义 2, ∗
1 浙江师范大学化学与生命科学学院 , 浙江金华 321000; 2 浙江省农业科学院作物与核技术利用研究所 , 浙江杭州 310021; 3 杭州师 范大学生命与环境科学学院 , 浙江杭州 310036; 4 西北农林科技大学 , 陕西杨凌 712100 摘
要 : 利用新版 SG 遗传图谱和 282 个 SG-DH 株系在中国西安、中国杭州和德国哥廷根 3 个生长环境下 8 个发育 时期测定的株高数据 , 运用 WinQTLCart2.5 复合区间作图法以及结合条件遗传分析方法对其进行静态和动态 QTL 分 析。结果显示 , 来自品种 Gaoyou 等位基因在 PHA3 和 PHC6 两个 QTL 上同时存在时 , 可降低株高约 20 cm; 而当植 株整合来自冬性品种 Sollux 的 PHA9 、 PHC1 和来自半冬性品种 Gaoyou 的 PHA1 、 PHA3 、 PHC6 时 , 株高可相应下 降 40 cm; 环境对株高 QTL 的作用机制影响不大 , 但不同 QTL 的基因表达模式不同 , 存在来自双亲之一的等位基因 控制株高和双亲等位基因在不同生长时期交替控制株高 2 种情况 ; 通常株高 QTL 在中后期才能被检测到 , 但基因多 在生长最为旺盛的短时期内表达 , 符合基因表达在先 , 性状表现在后的规律。 解析株高性状在不同发育时期基因的累 加效应和特定时段内的净表达效应 , 对克隆油菜株高基因和指导生产实践均有指导意义。
关键词 :
油菜 ; 株高 ; 发育时期 ; 静态 QTL; 动态 QTL Dynamic Analysis of QTL for Plant Height of Rapeseed at Different Develop- mental Stages XIE Tian-Tian 1,2 , CHEN Yu-Bo 2,3 , HUANG Ji-Xiang 2 , ZHANG Yao-Feng 2 , XU Ai-Xia 4 , CHEN Fei 2 , NI Xi-Yuan 2 , and ZHAO Jian-Yi 2, ∗
1 College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321000, China; 2 Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
3 College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China; 4 College of Agronomy, Northwest Agricultural and forestry University, Yangling 712100, China Abstract: The main objective of this study was to explore the genetic control mechanism of plant height in rapeseed and to reveal its dynamic gene expression in specific growing period. Taking advantage of the updated SG map, we determined phenotypic data of plant height from 282 SG-DH lines in three environments and eight development stages, and analyzed both static and dynamic quantitative trait locus (QTL) with unconditional and conditional mapping approach. The results showed that when alleles from Gaoyou existed in two major QTLs PHA3 and PHC6 synchronously, the plant height could decrease about 20 cm; when alleles of plant height came from Sollux in PHA9 and PHC1 ,
and Gaoyou in PHA1 , PHA3 ,
and PHC6 , the plants
fell 40 cm in height. The mechanism of QTL for plant height was less affected by environment, but various QTLs showed difference in gene expression patterns: plant height was dominated by alleles from one of parents or from two parents by turns at different stages. In general, the QTL for plant height could be detected from the middle to late stages, while the gene expression appeared shortly and only during the most active growing period, in congruent with the rule of “trait appearance goes after genetic expression”. Keywords:
Brasscia napus L . ; Plant height; Developmental stage; Static QTL; Dynamic QTL 株高是油菜重要农艺性状之一 , 与植株抗倒性 有关 [1] 。因倒伏造成的减产据报道可高达 10%~30% ( 严重时可达 50% 以上 ), 含油量亦比正常油菜低 10%~30% [2-3] 。 另根据机械化收割需求 , 普遍认为植 |
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