摘要：

本发明提供了一种四溴双酚A衍生物TBBPADHEE半抗原合成方法及用途,合成步骤如下:1,以4,4双(4羟苯基)戊酸为原料与甲醇发生酯化反应生成4,4双(4羟基苯基)戊酸甲酯;2,通过化合物a与液溴发生取代反应生成4,4双(3,5二溴4羟苯基)戊酸甲酯;3,4,4双(3,5二溴4羟基苯基)戊酸甲酯通过与2溴乙醇发生取代反应,得到半抗原4,4双(3,5二溴4(2羟基乙氧基)苯基)戊酸甲酯;4,化合物c通过与氢氧化钠发生皂化反应,盐酸酸化,得到半抗原4,4双(3,5二溴4(2羟基乙氧基)苯基)戊酸. The present invention provides a derivative of tetrabromobisphenol A TBBPA-DHEE hapten synthetic methods and uses synthetic procedures are as follows: 1, 4,4-bis (4-hydroxyphenyl) pentanoic acid as a raw material ester with methanol reaction of 4,4-bis (4-hydroxyphenyl) pentanoate; 2, reaction of a substituted 4,4-bis (3,5-dibromo compound by liquid bromine occurs with a 4-hydroxyphenyl ) pentanoate; 3,4,4-bis (3,5-dibromo-4-hydroxyphenyl) pentanoate and 2-bromoethanol by substitution reaction, to give the hapten 4,4-bis ( 3,5-dibromo-4- (2-hydroxyethoxy) phenyl) pentanoate; 4, compound c by saponification reaction with sodium hydroxide, acidified with hydrochloric acid to give the hapten 4,4-bis ( 3,5-dibromo-4- (2-hydroxyethoxy) phenyl) pentanoic acid. 该方法不仅简单,安全,可行,而且所得半抗原能与载体蛋白成功结合得到四溴双酚A衍生物TBBPADHEE的人工抗原. This method is not only simple, safe, feasible, and the resulting hapten bound to a carrier protein can be successfully obtained tetrabromobisphenol A derivative TBBPA-DHEE artificial antigen.

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