MSCV |
您所在的位置：网站首页 › pcdh载体多大 › MSCV |
|
载体名称: MSCV-IRES-GFP 载体抗性: Ampicillin 载体长度: 6488 bp 载体类型: Viral Expression ; Packaging Vectors 复制子: ori 载体来源: Tannishtha Reya 拷贝数: High copy number 启动子: MSCV 5'引物: pLXSN5; pBABE5 3'引物: pCDH-rev (GCATTCCTTTGGCGAGAG) 载体标签: EGFP MSCV-IRES-GFP 载体载体图谱 MSCV-IRES-GFP6488 bp300600900120015001800210024002700300033003600390042004500480051005400570060006300AmpRAmpR promoter5' LTRMESV Psigag (truncated)IRESEGFP3' LTRlac operatorlac promoterCAP binding siteori MSCV-IRES-GFP 载体载体序列 Copy Sequence 下载GeneBank文件(.gb) LOCUS 40924_2064 6488 bp DNA circular SYN 16-NOV-2021 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6488) TITLE Direct Submission REFERENCE 2 (bases 1 to 6488) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..6488 /mol_type="other DNA" /organism="synthetic DNA construct" CDS complement(458..1315) /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(1316..1420) /label=AmpR promoter LTR 2248..2764 /label=5' LTR /note="5' long terminal repeat from murine embryonic stem cell virus" misc_feature 2828..3169 /label=MESV Psi /note="packaging signal of murine embryonic stem cell virus" CDS 3236..3652 /codon_start=1 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" /translation="GQTVTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEWPTF NVGWPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVHPKP PPPLPPSAPSLPLEPPRSTPPRSSLYPALTPSLGA" misc_feature 3685..4246 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" CDS 4249..4965 /codon_start=1 /label=EGFP /note="enhanced GFP" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" LTR 5130..5644 /label=3' LTR /note="3' long terminal repeat from murine embryonic stem cell virus" protein_bind complement(5806..5822) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(5830..5860) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(5875..5896) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(join(6184..6488,1..284)) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"我们不提供该载体，载体信息仅供查询和参考。 |
今日新闻 |
推荐新闻 |
| CopyRight 2018-2019 办公设备维修网 版权所有 豫ICP备15022753号-3 |