Sample GSM78740
Query DataSets for GSM78740
Status
Public on Oct 20, 2005
Title
AR28 vs Tigr/Astra Reference 2
Sample type
genomic
Channel 1
Source name
1ug Tigr genomic DNA + 1ug Astra genomic DNA
Organism
Helicobacter pylori
Characteristics
1ug Tigr genomic DNA + 1ug Astra genomic DNA
Extracted molecule
genomic DNA
Label
Cy3
Channel 2
Source name
2 ug AR28 genomic DNA
Organism
Helicobacter pylori
Characteristics
2 ug AR28 genomic DNA
Extracted molecule
genomic DNA
Label
Cy5
Description
Simple annotation: Genomic DNA, Strain ComparisonImage: http://smd.stanford.edu/MicroArray/gifs/2000-12/11921.gif
Data processing
VALUE is Log (base 2) of the ratio of the mean of Channel 2 (usually 635 nm) to Channel 1 (usually 532 nm)
Submission date
Oct 19, 2005
Last update date
Feb 20, 2008
Organization
Stanford Microarray Database (SMD)
E-mail(s)
[email protected]
Phone
650-498-6012
URL
http://genome-www5.stanford.edu/
Department
Stanford University, School of Medicine
Street address
300 Pasteur Drive
City
Stanford
State/province
CA
ZIP/Postal code
94305
Country
USA
Platform ID
GPL15
Series (1)
GSE3479
Helicobacter pylori bacterial populations
Data table header descriptions
ID_REF
ID_REF
CH1I_MEAN
Mean feature pixel intensity at wavelength 532 nm.; Type: integer; Scale: linear_scale
CH2I_MEAN
Mean feature pixel intensity at wavelength 635 nm.; Type: integer; Scale: linear_scale
CH1B_MEDIAN
The median feature background intensity at wavelength 532 nm.; Type: integer; Scale: linear_scale; Channel: Cy3 Channel; Background
CH2B_MEDIAN
The median feature background intensity at wavelength 635 nm.; Type: integer; Scale: linear_scale; Channel: Cy5 channel; Background
CH1D_MEAN
The mean feature pixel intensity at wavelength 532 nm with the median background subtracted.; Type: integer; Scale: linear_scale; Channel: Cy3 Channel
CH2D_MEAN
.The mean feature pixel intensity at wavelength 635 nm with the median background subtracted.; Type: integer; Scale: linear_scale; Channel: Cy5 channel
CH1I_MEDIAN
Median feature pixel intensity at wavelength 532 nm.; Type: integer; Scale: linear_scale
CH2I_MEDIAN
Median feature pixel intensity at wavelength 635 nm.; Type: integer; Scale: linear_scale
CH1B_MEAN
The mean feature background intensity at wavelength 532 nm.; Type: integer; Scale: linear_scale; Background
CH2B_MEAN
The mean feature background intensity at wavelength 635 nm.; Type: integer; Scale: linear_scale; Background
CH1D_MEDIAN
The median feature pixel intensity at wavelength 532 nm with the median background subtracted.; Type: integer; Scale: linear_scale
CH2D_MEDIAN
The median feature pixel intensity at wavelength 635 nm with the median background subtracted.; Type: integer; Scale: linear_scale
CH1_PER_SAT
The percentage of feature pixels at wavelength 532 nm that are saturated.; Type: integer; Scale: linear_scale
CH2_PER_SAT
The percentage of feature pixels at wavelength 635 nm that are saturated.; Type: integer; Scale: linear_scale
CH1I_SD
The standard deviation of the feature intensity at wavelength 532 nm.; Type: integer; Scale: linear_scale; Channel: Cy3 Channel
CH2I_SD
The standard deviation of the feature pixel intensity at wavelength 635 nm.; Type: integer; Scale: linear_scale; Channel: Cy5 channel
CH1B_SD
The standard deviation of the feature background intensity at wavelength 532 nm.; Type: float; Scale: linear_scale; Channel: Cy3 Channel; Background
CH2B_SD
The standard deviation of the feature background intensity at wavelength 635 nm.; Type: integer; Scale: linear_scale; Channel: Cy5 channel; Background
PERGTBCH1I_1SD
The percentage of feature pixels with intensities more than one standard deviation above the background pixel intensity, at wavelength 532 nm.; Type: integer; Scale: linear_scale
PERGTBCH2I_1SD
The percentage of feature pixels with intensities more than one standard deviation above the background pixel intensity, at wavelength 635 nm.; Type: integer; Scale: linear_scale
PERGTBCH1I_2SD
The percentage of feature pixels with intensities more than two standard deviations above the background pixel intensity, at wavelength 532 nm.; Type: integer; Scale: linear_scale
PERGTBCH2I_2SD
The percentage of feature pixels with intensities more than two standard deviations above the background pixel intensity, at wavelength 532 nm.; Type: integer; Scale: linear_scale
SUM_MEAN
The sum of the arithmetic mean intensities for each wavelength, with the median background subtracted.; Type: integer; Scale: linear_scale
SUM_MEDIAN
The sum of the median intensities for each wavelength, with the median background subtracted.; Type: integer; Scale: linear_scale
RAT1_MEAN
Ratio of the arithmetic mean intensities of each spot for each wavelength, with the median background subtracted. Channel 1/Channel 2 ratio, (CH1I_MEAN - CH1B_MEDIAN)/(CH2I_MEAN - CH2B_MEDIAN) or Green/Red ratio.; Type: float; Scale: linear_scale
RAT2_MEAN
The ratio of the arithmetic mean intensities of each feature for each wavelength, with the median background subtracted.; Type: float; Scale: linear_scale
RAT2_MEDIAN
The ratio of the median intensities of each feature for each wavelength, with the median background subtracted.; Type: float; Scale: linear_scale
PIX_RAT2_MEAN
The geometric mean of the pixel-by-pixel ratios of pixel intensities, with the median background subtracted.; Type: float; Scale: linear_scale
PIX_RAT2_MEDIAN
The median of pixel-by-pixel ratios of pixel intensities, with the median background subtracted.; Type: float; Scale: linear_scale
RAT2_SD
The geometric standard deviation of the pixel intensity ratios.; Type: float; Scale: linear_scale
TOT_SPIX
The total number of feature pixels.; Type: integer; Scale: linear_scale
TOT_BPIX
The total number of background pixels.; Type: integer; Scale: linear_scale
REGR
The regression ratio of every pixel in a 2-feature-diameter circle around the center of the feature.; Type: float; Scale: linear_scale
CORR
The correlation between channel1 (Cy3) & Channel 2 (Cy5) pixels within the spot, and is a useful quality control parameter. Generally, high LOG_RAT2N_MEDIANs imply better fit & good spot quality.; Type: float; Scale: linear_scale
DIAMETER
The diameter in um of the feature-indicator.; Type: integer; Scale: linear_scale
X_COORD
X-coordinate of the center of the spot-indicator associated with the spot, where (0,0) is the top left of the image.; Type: integer; Scale: linear_scale
Y_COORD
Y-coordinate of the center of the spot-indicator associated with the spot, where (0,0) is the top left of the image.; Type: integer; Scale: linear_scale
TOP
Box top: int(((centerX - radius) - Xoffset) / pixelSize).; Type: integer; Scale: linear_scale
BOT
Box bottom: int(((centerX + radius) - Xoffset) / pixelSize).; Type: integer; Scale: linear_scale
LEFT
Box left: int(((centerY - radius) - yoffset) / pixelSize).; Type: integer; Scale: linear_scale
RIGHT
Box right: int(((centerY + radius) - yoffset) / pixelSize); Type: integer; Scale: linear_scale
FLAG
The type of flag associated with a feature: -100 = user-flagged null spot; -50 = software-flagged null spot; 0 = spot valid.; Type: integer; Scale: linear_scale
CH2IN_MEAN
Normalized LOG_RAT2N_MEDIAN of mean Channel 2 (usually 635 nm) intensity (CH2I_MEAN/Normalization factor).; Type: integer; Scale: linear_scale; Channel: Cy5 channel
CH2BN_MEDIAN
Normalized LOG_RAT2N_MEDIAN of median Channel 2 (usually 635 nm) background (CH2B_MEDIAN/Normalization factor).; Type: integer; Scale: linear_scale; Channel: Cy5 channel; Background
CH2DN_MEAN
Normalized LOG_RAT2N_MEDIAN of mean Channel 2 (usually 635 nm) intensity with normalized background subtracted (CH2IN_MEAN - CH2BN_MEDIAN).; Type: integer; Scale: linear_scale; Channel: Cy5 channel
RAT2N_MEAN
Type: float; Scale: linear_scale
CH2IN_MEDIAN
Normalized LOG_RAT2N_MEDIAN of median Channel 2 (usually 635 nm) intensity (CH2I_MEDIAN/Normalization factor).; Type: integer; Scale: linear_scale
CH2DN_MEDIAN
Normalized LOG_RAT2N_MEDIAN of median Channel 2 (usually 635 nm) intensity with normalized background subtracted (CH2IN_MEDIAN - CH2BN_MEDIAN).; Type: integer; Scale: linear_scale
RAT1N_MEAN
Ratio of the means of Channel 1 (usually 532 nm) intensity to normalized Channel 2 (usually 635 nm) intensity with median background subtracted (CH1D_MEAN/CH2DN_MEAN). Channel 1/Channel 2 ratio normalized or Green/Red ratio normalized.; Type: float; Scale: linear_scale
RAT2N_MEDIAN
Channel 2/Channel 1 ratio normalized, RAT2_MEDIAN/Normalization factor or Red/Green median ratio normalized.; Type: float; Scale: linear_scale
VALUE
same as UNF_VALUE but with flagged values removed
LOG_RAT2N_MEDIAN
Log (base 2) of the ratio of the median of Channel 2 (usually 635 nm) to Channel 1 (usually 532 nm) [log (base 2) (RAT2N_MEDIAN)].; Type: float; Scale: log_base_2
UNF_VALUE
Log (base 2) of the ratio of the mean of Channel 2 (usually 635 nm) to Channel 1 (usually 532 nm) [log (base 2) (RAT2N_MEAN)].; Type: float; Scale: log_base_2
Data table
ID_REF
CH1I_MEAN
CH2I_MEAN
CH1B_MEDIAN
CH2B_MEDIAN
CH1D_MEAN
CH2D_MEAN
CH1I_MEDIAN
CH2I_MEDIAN
CH1B_MEAN
CH2B_MEAN
CH1D_MEDIAN
CH2D_MEDIAN
CH1_PER_SAT
CH2_PER_SAT
CH1I_SD
CH2I_SD
CH1B_SD
CH2B_SD
PERGTBCH1I_1SD
PERGTBCH2I_1SD
PERGTBCH1I_2SD
PERGTBCH2I_2SD
SUM_MEAN
SUM_MEDIAN
RAT1_MEAN
RAT2_MEAN
RAT2_MEDIAN
PIX_RAT2_MEAN
PIX_RAT2_MEDIAN
RAT2_SD
TOT_SPIX
TOT_BPIX
REGR
CORR
DIAMETER
X_COORD
Y_COORD
TOP
BOT
LEFT
RIGHT
FLAG
CH2IN_MEAN
CH2BN_MEDIAN
CH2DN_MEAN
RAT2N_MEAN
CH2IN_MEDIAN
CH2DN_MEDIAN
RAT1N_MEAN
RAT2N_MEDIAN
VALUE
LOG_RAT2N_MEDIAN
UNF_VALUE
1
5789
5001
655
902
5134
4099
null
null
707
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null
null
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96
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82
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0
5556
1002
4554
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1.127
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2
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611
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98
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87
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0
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null
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3
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595
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3192
2657
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608
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1.201
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80
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null
82
92
112
122
0
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2952
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null
1.081
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-.113
null
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586
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711
481
null
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608
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null
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84
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1.478
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32
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82
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141
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0
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1.331
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314
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717
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37
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32
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82
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165
172
0
1223
906
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1.01
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null
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null
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6
5580
3529
654
821
4926
2708
null
null
670
833
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null
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100
100
null
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1.819
.55
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80
652
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null
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null
81
92
183
194
0
3921
912
3009
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null
1.637
null
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null
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7
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776
922
407
275
null
null
926
1021
null
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null
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null
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null
null
25
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1.48
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12
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83
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212
217
0
1330
1024
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1.33
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-.411
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8
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1125
645
882
437
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883
1070
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1.798
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237
242
0
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0
5152
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4192
1.025
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630
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710
649
null
null
648
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68
62
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1.094
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1.138
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32
160
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285
291
0
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1.015
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593
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768
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34
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1.181
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57
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80
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329
339
0
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410
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-2.52
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2.394
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0
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92
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1.104
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null
9
9
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null
2.235
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null
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null
32
220
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null
80
87
404
411
0
1251
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166
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null
2.006
null
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null
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754
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null
694
1009
null
null
null
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null
null
null
65
50
null
null
null
null
1.09
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null
null
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null
32
220
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null
null
null
80
87
427
434
0
1856
1087
769
1.02
null
null
.98
null
.028
null
.028
17
1729
1915
642
976
1087
939
null
null
692
1010
null
null
null
null
null
null
null
null
63
71
null
null
null
null
1.158
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null
null
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null
52
400
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null
null
79
88
450
459
0
2127
1084
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null
1.042
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-.06
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18
1565
1616
640
890
925
726
null
null
675
911
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null
null
null
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null
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null
82
82
null
null
null
null
1.274
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null
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null
52
340
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null
null
null
107
115
67
75
0
1795
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807
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null
null
1.146
null
-.197
null
-.197
19
1891
1907
662
893
1229
1014
null
null
717
928
null
null
null
null
null
null
null
null
96
93
null
null
null
null
1.212
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null
null
.762
null
32
176
.817
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null
null
null
107
113
91
97
0
2118
992
1126
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null
null
1.091
null
-.126
null
-.126
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1102
1197
627
832
475
365
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645
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null
1.301
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null
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236
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113
115
122
0
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null
null
1.17
null
-.226
null
-.226
Total number of rows: 4608 Table truncated, full table size 1166 Kbytes. Supplementary data files not provided
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