Neuronal Cell Culture Media and Astrocyte Medium |
您所在的位置:网站首页 › neurobasal培养基和DMEM › Neuronal Cell Culture Media and Astrocyte Medium |
Brain tissue comprises numerous cell types, including neurons and glial cells such as astrocytes. Primary neural cell preparations often contain a mixture of these cell types, so it is important to use the proper neural cell culture media and supplements to select for and enhance the desired population. Gibco Neuronal Medium and Astrocyte Medium are optimized to support cell growth as well as maintenance of cell viability, normal phenotype, and appropriate neural cell function. With these optimized neural cell culture media, neuronal and astrocytic populations can be maintained for weeks. Neuronal cell culture mediaThermo Fisher Scientific offers several varieties of neuronal media depending on your specific cell type and application: Neurobasal Plus neuronal medium—for long-term maintenance of embryonic and pre-natal neuronsNeurobasal-A neuronal medium—for long-term maintenance of adult and post-natal neuronsHibernate media—for short-term maintenance of neurons and viable brain tissue in ambient air (0% CO2) conditionsNeurobasal Neuronal Medium has been used and trusted by neuroscientists for over 20 years. This neuronal medium supports viability and health of pure populations of neurons in vitro without the need for an astrocyte feeder layer. Which selective media is used for neurons in culture?Neurobasal neuronal medium is one of the most popular culture media used for selective growth of neurons in vitro as it suppresses glial growth [1]. Neurobasal Plus neuronal medium, when used with B-27 Plus supplement, enhances survival of pre-natal and fetal neuronal cell cultures and enriches neurons in mixed neural cell cultures. Astrocyte mediaAstrocyte Medium is formulated for the growth and maintenance of human and rat astrocytes while retaining their phenotype. This astrocyte growth medium has three components: basal medium (DMEM), N-2 Supplement, and One Shot Fetal Bovine Serum (FBS). If more rapid proliferation is desired, addition of epidermal growth factor (EGF) is recommended, although cells may exhibit morphological and phenotypic changes. Neural cell culture media selection guideCell type Specific cell type/application Recommended media Astrocytes Rat astrocytesAstrocyte Medium Neurons Analysis of post-natal and adult neurons where the presence of phenol red is undesired Neurobasal-A Medium, minus phenol red Analysis of pre-natal and fetal neurons where the presence of phenol red is undesired Neurobasal Medium, minus phenol red Electrophysiology studies Neurobasal Plus Medium Glucose-response or insulin receptor studies in post-natal neurons where the presence of D-glucose or sodium pyruvate may interfere Neurobasal-A, minus D-glucose and Sodium Pyruvate Growth and maintenance of pre-natal and fetal neurons Neurobasal Plus Medium Maintenance/maturation of post-natal and adult brain neurons Neurobasal-A Medium Maintenance/maturation of stem cell-derived neurons Neurobasal Plus Medium Manipulation of embryonic neurons at ambient CO2 for at least 48 hours while retaining viability Hibernate-E Medium Manipulation of post-natal neurons at ambient CO2 for at least 48 hours while retaining viability Hibernate-A Medium References1. Brewer, G.J.; Torricelli, J.R.; Evege, E.K.; Price, P.J. J Optimized survival of hippocampal neurons in B27-supplemented Neurobasal, a new serum-free medium combination. Neurosci Res. 1993 Aug 1;35(5):567-76. doi: 10.1002/jnr.490350513. Support and resources for neuronal cell culture media Poster: An advanced media system for improved neuronal viability and function posterPoster: Regulated control of glial cell outgrowth in primary rodent neuronal culturesNeural Cell Culture ProtocolsPrimary Cell Culture Support—Getting StartedNeurobiology HandbookContact Technical SupportSend us a Services Inquiry |
今日新闻 |
推荐新闻 |
CopyRight 2018-2019 办公设备维修网 版权所有 豫ICP备15022753号-3 |