GB/T 22249-2008Determination of lycopene in health foodsICS 67.040C53National Standards of People's Republic of ChinaHealth food Determination of LycopenePosted 2008-07-312008-11-01 implementationPeople's Republic of China Ministry of HealthStandardization Administration of China releasedForewordThis standard is proposed and administered by the People's Republic of China Ministry of Health.This standard is drafted by. China Center for Disease Control Nutrition and Food Safety.Participated in the drafting of this standard. Beijing Center for Disease Control and Prevention.The main drafters of this standard. Yang Jin, Ruggie, Wang Zhu days, Song Fengying, Luo Ren only.This standard is the first release.Health food Determination of Lycopene1 ScopeThis standard specifies the method for determination of lycopene in health foods.This standard applies to the effectiveness as a main ingredient in health food added to the determination of lycopene.When the sampling amount of 1.0g, set the volume to 10mL, injection volume 10μL, the limit of detection (LOD) of 3 × 10-4g/Kg, the limit of quantitation (LOQ) for the1 × 10-3g/kg. The linear range of 0.200μg/mL ~ 20.0μg/mL.Principle 2According to the physical and chemical properties of lycopene soluble in solvents such as methylene chloride, the sample was Coke sexual gallic acid - methylene chloride solution was extracted, volume,Backward filter performance liquid chromatography, after reverse phase chromatography by UV detector, retention time and peak area based on the qualitative andQuantified.3 Reagents and materials3.1 acetonitrile (CH3CN). chromatography.3.2 methanol (CH3OH). chromatography.3.3 dichloromethane (CH2Cl2). AR.3.4 Coke sexual gallic acid (C6H3 (OH) 3). AR.3.5 N, N- dimethylformamide (C3H7NO). AR.3.6 lycopene reference. Purity ≥95% (from light at -70 ℃ refrigerator).3.7 Coke sexual gallic acid - methylene chloride solution. Weigh 5g Coke sexual gallic acid dissolved in methylene chloride and dilute to 100mL.3.8 lycopene reference solution. accurately weighed reference substance lycopene 1mg (accurate to 0.0001g), placed in 10mL brown capacityBottle, add coke gallic acid - methylene chloride solution to dissolve and dilute to volume, and mix this with the existing temporary solution. Because lycopene instabilitySet before use to liquid chromatography to determine the purity of normalization.4 instruments and equipment4.1 High performance liquid chromatography. a UV detector.4.2 ultrasonic cleaner.Step 5 Analysis5.1 Preparation of the sample5.1.1 General sample preparationAccording to the sample content of lycopene, weighed 0.5g ~ 2.0g homogeneous sample (accurately weighed to 0.001g) placed 25mL brownFlask, add coke gallic acid - methylene chloride solution 20mL, ultrasonic extraction 30min, plus coke gallic acid - methylene chloride solutionTo the mark, shake, too 0.45μm filters. The filtrate was set aside.5.1.2 Preparation of microencapsulated sampleAccording to the sample content of lycopene, weighed 0.5g ~ 2.0g homogeneous sample (accurately weighed to 0.001g) placed 25mL brownFlask, add 0.2g Coke sexual gallic acid and 5mLN, after N- dimethylformamide ultrasonic extraction 30min, then coke gallic...