PE anti

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PE anti

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Pricing & Availability Product Details Antigen Details Documentation Reviews Related Protocols Related Products Related Pages & Pathways Related FAQs Other Formats Concentration & Expiration Lookup Certificate of Analysis Pricing & Availability Clone AY13 (See other available formats) Regulatory Status RUO Other Names Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, HER1 Isotype Mouse IgG1, κ Ave. Rating Submit a Review Product Citations publications AY13_PE_090111

Human cervical cancer cell line HELA was stained with EGFR (clone AY13) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram). AY13_PE_090111

Human cervical cancer cell line HELA was stained with EGFR (clone AY13) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram). Compare all formats See PE spectral data Cat # Size Price Quantity Check Availability Save Input string was not in a correct format. 352903 25 tests £81 Input string was not in a correct format. 352904 100 tests £221 Description

Epidermal growth factor receptor (EGFR) is a transmembrane glycoprotein and member of the protein kinase superfamily that regulates cell growth and differentiation. EGFR binds EGF, TGF-α, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30, and vaccinia virus growth factor - all members of the EGF family. Ligand binding induces EGFR dimerization and autophosphorylation, initiating the MAPK, Akt, and JNK signaling pathways. EGFR is expressed by epithelial and endothelial cells and is frequently expressed by epithelial carcinomas.

Product Details Technical Data Sheet (pdf) Product Details Verified Reactivity Human Antibody Type Monoclonal Host Species Mouse Immunogen Non-small cell lung cancer (NSCLC) cell line NCI-H322 Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) Preparation The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. Concentration Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.) Storage & Handling The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm) Application References

(PubMed link indicates BioLegend citation)

Yamaguchi M, et al. 2009. The 15th Annual Meeting Japan Society of Gene Therapy. p1056. Abstract 92. Product Citations Wang Y, et al. 2014. Biomaterials. 35:4297. PubMed Sreevalsan S, et al. 2020. EMBO Rep. 21:e50155. PubMed Kitamura Y, et al. 2021. Sci Adv. 7: . PubMed Halim L, et al. 2017. Cell Rep. 10.1016/j.celrep.2017.06.079. PubMed Bak R and Proteus M. 2017. Cell Rep. 10.1016/j.celrep.2017.06.064. PubMed Wang JL, et al. 2022. Cancers (Basel). 14:. PubMed Lu T, et al. 2022. Nat Commun. 13:2576. PubMed Jayasinghe MK, et al. 2022. Theranostics. 12:3288. PubMed Aldeghaither DS, et al. 2019. Cancer Immunol Res. 7:230. PubMed Carnevale J, et al. 2022. Nature. 609:174. PubMed Grass G, et al. 2013. J Biol Chem. 288:26089. PubMed Ahn S, et al. 2019. Cancer Immunol Res. 0.828472222. PubMed Edinger N, et al. 2016. PLoS One. 11: 0162321. PubMed Wing A, et al. 2018. Cancer Immunol Res. 6:605. PubMed Okada R, et al. 2021. EBioMedicine. 67:103345. PubMed Han X, et al. 2017. Mol Ther. 10.1016/j.ymthe.2017.07.009. PubMed Brohl AS, et al. 2021. Cell Rep. 37:110047. PubMed Jarantow S, et al. 2015. J Biol Chem. 290: 24689 - 24704. PubMed Li G, et al. 2021. Mol Ther Oncolytics. 22:507. PubMed Fischer A, et al. 2020. Toxins (Basel). 12:00. PubMed Quijano-Rubio A, et al. 2022. Nat Biotechnol. Online ahead of print. PubMed RRID AB_10898161 (BioLegend Cat. No. 352903) AB_10896794 (BioLegend Cat. No. 352904) Antigen Details Structure Member of the protein kinase superfamily; transmembrane glycoprotein; ligand binding induces dimerization and autophosphorylation Distribution

Epithelial cells and endothelial cells; frequently found in epithelial carcinomas

Function Controls cell growth and differentiation Interaction MAPK, Akt, JNK Ligand/Receptor Members of the epidermal growth factor (EGF) family such as EGF, TGF-α, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor Cell Type Endothelial cells, Epithelial cells Biology Area Cell Biology, Cell Cycle/DNA Replication, Immunology, Innate Immunity, Neuroscience, Synaptic Biology Molecular Family CD Molecules, Growth Factors Antigen References

1. da Cunha Santos G, et al. 2011. Annu. Rev. Pathol. 6:49. 2. Gusterson BA and Hunter KD. 2009. Lancet Oncol. 10:522. 3. Mano M and Humblet Y. 2008. Nat. Clin. Pract. Oncol. 5:415. 4. Pao W and Chmielecki J. 2010. Nat. Rev. Cancer 10:760.

Gene ID 1956 View all products for this Gene ID Specificity (DOES NOT SHOW ON TDS): EGFR Specificity Alt (DOES NOT SHOW ON TDS): EGFR UniProt View information about EGFR on UniProt.org Documentation Technical Data Sheet (pdf) Certificate of Analysis Safety Data Sheet Reviews Review This Product Related Protocols Cell Surface Flow Cytometry Staining Protocol Related Products Description Clone Applications Related Pages & Pathways Pathways EGF Pathway ErbB Family Pathway ERK Signaling FAK1 Pathway p38 Signaling Pages

Quality Control

Multicolor Staining Guide

Fluorescence Spectra Analyzer

Fluorophore Brightness Index

Custom Panel Construction

Fluorophore Equivalency Tool Related FAQs What type of PE do you use in your conjugates? We use R-PE in our conjugates. Other Formats View All EGFR Reagents Request Custom Conjugation Description Clone Applications Purified anti-human EGFR AY13 FC,ICC PE anti-human EGFR AY13 FC APC anti-human EGFR AY13 FC Alexa Fluor® 488 anti-human EGFR AY13 FC,ICC Brilliant Violet 421™ anti-human EGFR AY13 FC,ICC PE/Cyanine7 anti-human EGFR AY13 FC PerCP/Cyanine5.5 anti-human EGFR AY13 FC Alexa Fluor® 594 anti-human EGFR AY13 ICC,IHC-P Alexa Fluor® 647 anti-human EGFR AY13 FC Brilliant Violet 711™ anti-human EGFR AY13 FC PE/Dazzle™ 594 anti-human EGFR AY13 FC TotalSeq™-A0132 anti-human EGFR AY13 PG Brilliant Violet 605™ anti-human EGFR AY13 FC APC/Fire™ 750 anti-human EGFR AY13 FC TotalSeq™-B0132 anti-human EGFR AY13 PG TotalSeq™-C0132 anti-human EGFR AY13 PG Biotin anti-human EGFR AY13 FC Concentration & Expiration Lookup Certificate of Analysis Customers Also Purchased PE anti-human CD340 (erbB2/HER-2) Clone: 24D2

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Go To Top Version: 1 Revision Date: 11/30/2012

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

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Compare Data Across All Formats

This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

Purified anti-human EGFR