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北京百奥创新代理cellscript在中国的业务。 cellscript北京代理,cellscript华东代理,cellscript华北代理,cellscript华中代理,cellscript专业代理。 CELLSCRIPT™的使命是提供优质的产品和技术,用于制作和使用RNA进行细胞翻译,用于临床研究和治疗。目前的产品包括用于体外转录的试剂盒,使用帽类似物或加帽酶的5'RNA加帽,以及3'RNA聚腺苷酸化,以及用于制备加帽的聚(A)尾部mRNA用于翻译的一体化试剂盒。 在细胞中。 CELLSCRIPT™近推出了INCOGNITO™RNA转录试剂盒,用于体外合成含有修饰核苷的RNA,例如假尿苷(Ψ)和/或5-甲基胞苷(m5C)代替相应的U或C规范核苷(图1)。 这些试剂盒之所以如此命名,是因为带帽,聚腺苷酸化和核苷修饰的RNA产物(称为“INCOGNITO mRNAs")被wei装,因此它们在转染到表达的哺乳动物细胞时不会像相应的未修饰mRNA那样诱导先天免疫应答。 各种RNA传感器。 cellscript专业代理,部分产品列表: mRNA ProductionT7 mScript™ Standard mRNA Production System (PDF) Simply the best way to make in vitro mRNA. Combines the benefits of high-yield transcription with those of post-transcriptional capping and tailing all in one kit. See T7-FlashScribe Transcription Kit, ScriptCap m7G Capping System, ScriptCap 2'-O-Methyltransferase Kit and A-Plus Poly(A) Polymerase Tailing Kit on this page. C-MSC11610 10 reactions $320 C-MSC100625 25 reactions $702 In Vitro TranscriptionT7-FlashScribe™ Transcription Kit (PDF) For the production of in vitro transcribed RNA with T7 RNA polymerase and canonical (GAUC) NTPs. A reaction produces 180 µg RNA from 1 µg DNA in30 minutes. C-ASF3507 50 reactions $286 T7-Scribe™ Standard RNA IVT Kit (PDF) For the production of in vitro transcribed RNA with T7 RNA polymerase and canonical (GAUC) NTPs. A reaction produces 150 µg RNA from 1 µg DNA in 2 hours. C-AS3107 50 reactions $267 SP6-Scribe™ Standard RNA IVT Kit (PDF) For the production of in vitro transcribed RNA with SP6 RNA polymerase and canonical (GAUC) NTPs. A reaction produces 90 µg RNA from 1 µg DNA in 2 hours. C-AS3106 50 reactions $267 Post-Transcriptional Capping ScriptCap™ m7G Capping System (PDF) For post-transcriptional capping of in vitro transcribed RNA with ScriptCap Capping Enzyme, GTP and SAM. Cap 0 (N7-methyl-G) caps can be built with efficiencies approaching 100%. This cannot be accomplished with co-transcriptional capping methods. Use in conjunction with ScriptCap 2'-O-Methyltransferase to produceCap 1 caps, that which is found on most eukaryotic mRNAs. C-SCCE0625 25 reactions $436 ScriptCap™ 2'-O-Methyltransferase Kit (PDF) For conversion of RNA containing Cap 0 caps to those containing Cap 1 caps, which are found on most eukaryotic mRNAs. Can be used simultaneously with ScriptCap m7G Capping System to produce virtually 100% Cap 1-capped RNA in a single reaction. C-SCMT0625 25 reactions $219 ScriptCap™ Cap 1 Capping System (PDF) For post-transcriptional capping of in vitro transcribed RNA with ScriptCap Capping Enzyme, ScriptCap 2'-O-Methyltransferase, GTP and SAM. Either Cap 0 or Cap 1 caps can be built with efficiencies approaching 100%. This cannot be accomplished with co-transcriptional capping methods. C-SCCS1710 10 reactions $211 Co-Transcriptional Capping MessageMAX™ T7 ARCA-Capped Message Transcription Kit (PDF) For co-transcriptional capping of IVT RNA with T7 RNA polymerase and Anti-Reverse cap analog (ARCA). A reaction produces 60 µg RNA from 1 µg DNA in 30 minutes. Reaction products are 80% correctly capped and 20% uncapped. C-MMA60710 10 reactions $323 AmpliCap-Max™ T7 High Yield Message Maker Kit (PDF) For co-transcriptional capping of IVT RNA with T7 RNA polymerase and standard cap analog. A reaction produces 60 µg RNA from 1 µg DNA in 30 minutes. Reaction products are 40% correctly capped, 40% incorrectly capped and 20% uncapped. C-ACM04037 25 reactions $368 AmpliCap™ SP6 High Yield Message Maker Kit (PDF) For co-transcriptional capping of IVT RNA with SP6 RNA polymerase and standard cap analog. A reaction produces 35 µg RNA from 1 µg DNA in 2 hours. Reaction products are 40% correctly capped, 40% incorrectly capped and 20% uncapped. C-AC0706 25 reactions $368 Post-Transcriptional TailingA-Plus™ Poly(A) Polymerase Tailing Kit (PDF) For post-transcriptional poly(A) tail addition to RNAs. Allows tailing of mRNAsin vitro, to lengths equivalent to or greater than those found in nature. This cannot be accomplished with co-transcriptional tailing methods. C-PAP5104H 50 reactions $219 Modified NTP In Vitro TranscriptionINCOGNITO™ T7 Ψ-RNA Transcription Kit (PDF) For the production of pseudouridine-containing, in vitro transcribed RNA with T7 RNA polymerase. Ψ-mRNAs induce lower innate immune responses in mammalian cells. A reaction produces 100 µg RNA from 1 µg DNA in 2 hours. C-ICTY110510 10 reactions $410 INCOGNITO™ SP6 Ψ-RNA Transcription Kit (PDF) For the production of pseudouridine-containing, in vitro transcribed RNA with SP6 RNA polymerase. Ψ-mRNAs induce lower innate immune responses in mammalian cells. A reaction produces 60 µg RNA from 1 µg DNA in 2 hours. C-ICSY110510 10 reactions $410 INCOGNITO™ T7 5mC- & Ψ-RNA Transcription Kit (PDF) For the production of 5-methylcytosine- pseudouridine-containing, in vitro transcribed RNA with T7 RNA polymerase. 5mC- Ψ-mRNAs induce lower innate immune responses in mammalian cells. A reaction produces 100 µg RNA from 1 µg DNA in 2 hours. C-ICTMY110510 10 reactions $691 INCOGNITO™ T7 ARCA 5mC- & Ψ-RNA Transcription Kit (PDF) For co-transcriptional capping of 5-methylcytosine- pseudouridine-containing,in vitro transcribed RNA with T7 RNA polymerase and Anti-Reverse cap analog (ARCA). 5mC- Ψ-mRNAs induce lower innate immune responses in mammalian cells. A reaction produces 40 µg RNA from 1 µg DNA in 3 hours. Reaction products are 80% correctly capped and 20% uncapped. C-ICTAMY110510 10 reactions $842 mRNA DecappingCap-Clip™ Acid Pyrophosphatase (PDF) For the removal of cap structures (decapping) from various RNAs (i.e. mRNAs) resulting in 5'-monophosphate RNAs. Can be substituted unit for unit, into any preexisting protocol for Tobacco Acid Pyrophosphatase (TAP) utilizing the same buffer. C-CC15011H 100 units $550 RNase Inhibition ScriptGuard™ RNase Inhibitor (PDF) Inhibits eukaryotic RNases A, B and C. Use as a safeguard against RNA degradation in any RNA-containing reaction. 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