Lane 1 : Human fetal vessel lysateLane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysateLane 3 : HUVEC (human umbilical vein endothelial cell line) whole cell lysateLane 4 : SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysateLane 5 : Neuro-2a (mouse neuroblastoma cell line) whole cell lysateLane 6 : bEND.3 (mouse brain endothelioma cell line) whole cell lysateLane 7 : C6 (rat glial tumor cell line) whole cell lysateLane 8 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate

Lysates/proteins at 20 µg per lane.

SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 27 kDaObserved band size: 40 kDa why is the actual band size different from the predicted?

Blocking/Dilution: 5% NFDM/TBST

Exposure time : Lanes 1-4/7-8: 3 minutes; Lane 5: 41 seconds; Lane 6: 15 seconds.

The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.