羽扇豆食品具有有益的健康益处。由于来自羽扇豆蛋白的胰蛋白酶和消化性肽调节HepG2细胞中的胆固醇代谢并增加LDL摄取，因此这项工作的目的是评估这些羽扇豆肽是否被人肠Caco-2细胞吸收。将细胞分化15天，并通过从顶侧温育每种羽扇豆肽混合物来进行转运实验。4小时后，收集基底外侧溶液并通过HPLC-Chip-MS / MS分析。在基底外侧样品中鉴定出了十一种胰蛋白酶和八种消化肽，它们来自羽扇豆贮藏蛋白。的体外实验显示，基底外侧肽维持其抑制3-羟基-3-甲基戊二酰辅酶A还原酶（HMGCoAR）活性和容量，在硅片对接模拟允许假设哪些肽可能更有效地与HMGCoAR催化位点结合。这是第一个提供证据证明具有特定结构的羽扇豆肽可能在人肠细胞中吸收的证据。

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Lupin foods provide useful health benefits. Since tryptic and peptic peptides from lupin protein modulate cholesterol metabolism in HepG2 cells and increase LDL-uptake, this work had the goal of assessing whether these lupin peptides are absorbed by human intestinal Caco-2 cells. Cells were differentiated for 15 days and transport experiments were performed by incubating each lupin peptide mixture from the apical side. After 4 h, basolateral solutions were collected and analysed by HPLC-Chip-MS/MS. Eleven tryptic and eight peptic peptides, deriving from lupin storage proteins, were identified in the basolateral samples. An in vitro assay showed that basolateral peptides maintain their capacity to inhibit 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCoAR) activity and in silico docking simulations permitted to hypothesise which peptides may bind more efficiently to the HMGCoAR catalytic site. This is the first investigation providing evidence that lupin peptides with specific structures are potentially absorbed in human intestinal cells.