阅读量：

133

作者：

宁张弛，宋志前，王淳，刘元艳，曾鸿莲，甘嘉荷，马新玲，刘振丽

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摘要：

目的:确定引起南北方醋乳香颜色差异的炮制因素,并分析对乳香主要6种乳香酸含量的影响.方法:收集不同地区市售醋乳香,分别采用文火和中火两种温度醋炙不同时间,得到不同醋乳香样品.直观观察醋乳香颜色,应用色差仪测定色度参数值,并采用高效液相色谱法测定6种主要乳香酸成分含量.采用Zorbax Extend-C18(4.6 mm×50 mm,1.8 μm)色谱柱,以乙腈-0.1%磷酸为流动相,梯度洗脱,流速1 mL· min.,检测波长210 nm,250 nm,柱温30℃.结果:直观观察表面颜色,北方醋乳香呈现黄色或棕黄色,南方则为棕褐色或黑褐色,显示炮制程度不同.色度参数值显示,市售北方和南方醋乳香的亮度L*值和红绿色度a*值差异显著,L*值分别为75.327-80.746和44.321-49.527,a*值分别为5.378-6.502和9.423-9.978,蓝黄色度b*值没有明显差异.直观观察同样表面颜色的醋乳香,L*值和a*值也有一定差异.自制醋乳香色度参数结果显示,随着炮制温度和时间变化,醋乳香外观颜色和色度参数值L*值和a*值发生改变.但乳香在文火条件下即使炒制30 min,也达不到南方醋乳香的色度参数值,而中火炒制11 min即可达到.含量测定结果显示,α-乳香酸,β-乳香酸,3-乙酰-α-乳香酸和3-乙酰-β-乳香酸4种成分含量升高,而11-羰基乳香酸,3-乙酰-11-羰基乳香酸在炮制后含量降低.中火升高或降低的幅度比文火大,而同一温度随炮制时间延长,含量也呈现逐渐升高或降低的趋势.结论:炮制温度是造成南北方醋乳香颜色差异的主要因素,并引起乳香中6种乳香酸含量升高或降低的幅度不同.色差仪色度参数值可客观表征醋乳香外观颜色.%This paper focused on factors which affected on different color of northern and southern region vinegar-processed frankincense.Meanwhile,contents of six main boswellic acids were also determined to elaborate the influence of heat in chemical components.Vinegar-processed frankincense from northern and southern region was collected.And different temperature and time were used in the processing of frankincense to receive the vinegar-processed frankincense samples.The color difference meter was utilized combining with the PCA statistic analysis method.The Zorbax ExtendC18 chromatographic column (4.6 mm × 50 mm,1.8 μm) was used with acetonitrile-0.1% phosphoric acid as the mobile phase and gradient elution.The velocity of flow was 1 mL· min-1.The detection wavelength was 210 nm and 250 nm.The column temperature was 30℃.The results showed that the color of northern region processed frankincense was yellow or pale brown.And the southern region processed frankincense was pale brown or dark brown.It showed the difference on processed degree.The L* value of the northern processed frankincense was 75.327 to 80.746 and the L* value of southern processed was 44.321 to 49.527.The a* value of the northern processed frankincense was 5.378 to 6.502 and the a* value of southern processed was 9.423 to 9.978.There was no significant difference on b*.There were certain differences on L* and a* among vinegar-processed frankincense with the same surface color.The color parameter results of self-made vinegar-processed frankincense indicated that along with changes of processing temperature and time,the color,L* and a* change.Even frankincense processed for 30 min with mild fire,it will not achieve the color parameter value of the southern region vinegar-processed frankincense.However,after 11 min processing with medium fire,the color can be achieved.The content determination results showed that four contents,including α-boswellic acid,β-boswellic acid,3-acetyl-α-boswellic acid and 3-acetyl-β-boswellic acid were increased.Contents of 11-carbonyl-3-boswellic acid and 3-acetyl-11-carbonyl-β-boswellic were decreased after being processed.The range of increasing or decreasing by medium fire was higher than mild fire.At the same temperature,as the increasing of processing time,the content has an increasing or decreasing tendency.It was concluded that temperature was the main factor influencing the color of vinegar-processed frankincense from northern and southern regions.Different processing degrees also make influence on the contents of chemical compounds.The color parameter value can be used to evaluate the color of processed frankincense.

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关键词：

醋乳香 颜色 乳香酸 炮制程度 色度参数值

年份：

2017