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下面是我们从网上搜集的最全的常用测序通用引物列表： 测序通用引物列表 引物名称序列(5'→3')描述3'AOX1GCAAATGGCATTCTGACATCCFor Pichia vectors with AOX1 terminator, reverse primer5'AOX1GACTGGTTCCAATTGACAAGCFor Pichia vectors with AOX1 promoter, forward primerPolyhedrin forwardAAATGATAACCATCTCGCPolyhedrin promoter, forward primerpGP704-RAACAAGCCAGGGATGTAACGR6K gamma origin, 3' of MCS in pGP704 vector, reverse primerSeqL-B (ATTL2)AACATCAGAGATTTTGAGACACBAC1AACCATCTCGCAAATAAATAPIRES3-EGFP-RAACGCACACCGGCCTTATTCUbx-FAACTCGTACTTTGAACAGGCDrosophila Ultrabithorax gene, forward primerPBV220FAAGAAGGGCAGCATTCAAAGpbd-gal4-rAAGAGTTACTCAAGAACAAGAApMRB101-FAAGATGCAGGCAGCTGAGTTHCMV major immediate-early protein (IE), forward primerPB42AD-RAAGCCGACAACCTTGATTGGAGT7EEVAAGGCTAGAGTACTTAATACGGal4-ADAATACCACTACAATGGAT3' end of Gal4 activation domain, forward primerGAL4-ADforAATACCACTACAATGGATGATG3' end of Gal4 activation domain, forward primerpBRforEcoAATAGGCGTATCACGAGGCIn pBR322, upsteam of EcoRI site, forward primerGAL1AATATACCTCTATACTTTAACGTCS. cerevisiae GAL1 promoter, forward primerNOSRAATCATCGCAAGACCGGCGW-5'AATCTCGCCGGATCCTAACT5' end of Gateway cassette, reverse primerAC5ACACAAAGCCGCTCCATCAGDrosophila Actin 5C promoter, forward primerT7(17base)ACATCCACTTTGCCTTTCTCRCAS-FACATGGGTGGTGGTATAGCGCTTGCG3' of Rous sarcoma virus (RSV) env gene, forward primerGST-TagACCCAATGTGCCTGGATGCGpBABE 3'ACCCTAACTGACACACATTCCSV40 enhancer, 3' of MCS in pBABE vectors, reverse primerV5 ReverseACCGAGGAGAGGGTTAGGGATV5 epitope, reverse primerpLNCX-RACCTACAGGTGGGGTCTTTCATTCCCBAC2ACGCACAGAATCTAGCGCTTpLTet-FACTGAGCACATCAGCAGGACLambda phage early leftward (pL) promoter, forward primerpCEP-FAGAGCTCGTTTAGTGAACCGF27AGAGTTTGATCCTGGCTCA3'ADAGATGGTGCACGATGCACAGL4440AGCGAGTCAGTGAGCGAG5' of MCS in L4440 vector, forward primerM13 Reverse (-48)AGCGGATAACAATTTCACACM13/pUC ReverseAGCGGATAACAATTTCACACAGGIn lacZ geneM13R(-48)AGCGGATAACAATTTCACACAGGAIn lacZ geneLNCXAGCTCGTTTAGTGAACCGTCAGATCHuman CMV promoter, forward primerpLNCX-FAGCTCGTTTAGTGAACCGTCAGATCGDsRed1-CAGCTGGACATCACCTCCCACAACG3' end of DsRed1, forward primer2.0rev primerAGGCGATTAAGTTGGGTACMV-ReverseAGTAGGAAAGTCCCGTAAGGLuc-FAGTCAAGTAACAACCGCGA3' end of luciferase, forward primerN-CMV-30ATAACCCCGCCCCGTTGAmp-RATAATACCGCGCCACATAGC5' end of ampicillin resistance gene, reverse primerMMLV-FATCAGTTCGCTTCTCGCTTCMoloney murine leukemia virus LTR (MoMuLV), forward primerpBAD ForwardATGCCATAGCATTTTTATCCFor vectors with E. coli araBAD promoter, forward primerpET-UpstreamATGCGTCCGGCGTAGAU6ATGGACTATCATATGCTTACCGTApENTR-RATGGCTCATAACACCCCTTG3' of attL2 in pENTR vector, reverse primerT3ATTAACCCTCACTAAAGGGAT3 promoter, forward primerpCMV5RATTATAGAGGACACCTAGTCpDsRed-C-3'ATTCATTTTATGTTTCAGGTTCASFFV-FATTGATTGACTGCCCACCTCSpleen focus forming virus 5' LTR, forward primerSP6ATTTAGGTGACACTATAGSP6 promoter, forward primerS1CAACGTGAAAAAATTATTATTCGCTRC-FCAAGGCTGTTAGAGAGATAATTGGAHuman U6 promoter, forward primerT3CAATTAACCCTCACTAAAGGAUG1 ForwardCAATTTACATCTTTATTTATTAACGFor Pichia vectors with AUG1 promoter, forward primerSV40-spliceRCACAAAGATCCGGACCAAAGSV40 splice sequence, reverse primerpIRES.RCACTGCATTCTAGTTGTGGTmU6-FCAGCACAAAAGGAAACTCACCMouse U6 promoter, forward primerMSCV-revCAGCGGGGCTGCTAAAGCGCATGCMurine stem cell virus, reverse primerM13 ReverseCAGGAAACAGCTATGACIn lacZ geneM13RCAGGAAACAGCTATGACCmU6CAGTGCAGGGGAAAGAATAGTAGACWPRE-RCATAGCGTAAAAGGAGCAACA5' end of WPRE, reverse primerMT ForwardCATCTCAGTGCAACTAAADrosophila metallothionein promoter, forward primerEGFP-CCATGGTCCTGCTGGAGTTCGTG3' end of EGFP, forward primermPGK-FCATTCTGCACGCTTCAAAAGMouse PGK promoter, forward primerpTRE 3'CCACACCTCCCCCTGAACPB42AD-FCCAGCCTCTTGCTGAGTGGAGATGhGH-PA-RCCAGCTTGGTTCCCAATAGAHuman growth hormone terminator, reverse primerRluc-FCCAGGATTCTTTTCCAATGC3' end of Renilla luciferase, forward primerpTarget.FCCAGGATTTTCCCAGTCACpCAMBIA1301-5'CCAGGCTTTACACTTTATGCGFP-RCCATCTAATTCAACAAGAATTGGGACAAC5' end of GFP, reverse primerM13/pUC ForwardCCCAGTCACGACGTTGTAAAACGIn lacZ genemCherry-FCCCCGTAATGCAGAAGAAGA3' end of mCherry, forward primerpQE promoterCCCGAAAAGTGCCACCTG5' of MCS in pQE vectors, forward primerIntein ForwardCCCGCCGCTGCTTTTGCACGTGAGM13(-96)CCCTCATAGTTAGCGTAACGMSCV|pLXSN 5'CCCTTGAACCTCCTCGTTCGACCMurine stem cell virus, forward primerpGEX 3'CCGGGAGCTGCATGTGTCAGAGG3' of MCS in pGEX vectors, reverse primerIRES-RCCTCACATTGCCAAAAGACG5' end of IRES, reverse primerSV40-pArevCCTCTACAAATGTGGTATGGLucNrevCCTTATGCAGTTGCTCTCC5' end of luciferase, reverse primerS.tagCGAACGCCAGCACATGGACACMV ForwardCGCAAATGGGCGGTAGGCGTGHuman CMV immediate early promoter, forward primerOpIE2 ForwardCGCAACGATCTGGTAAACACOpIE2 promoter, forward primerM13F（-47）CGCCAGGGTTTTCCCAGTCACGACpTRE 5'CGCCTGGAGACGCCATCCpBluescript SKCGCTCTAGAACTAGTGGATCpRS-markerCGGCATCAGAGCAGATTGTATo sequence yeast selectable marker in pRS vectorsGPDpro-FCGGTAGGTATTGATTGTAATTCTGS. cerevisiae GPD promoter, forward primerpLEXA-FCGTCAGCAGAGCTTCACCATLexACGTCAGCAGAGCTTCACCATTG3' end of LexA DNA binding domain, forward primerEGFP-NrevCGTCGCCGTCCAGCTCEGFP-NCGTCGCCGTCCAGCTCGACCAEGFP-NCGTCGCCGTCCAGCTCGACCAG5' end of EGFP, reverse primerPinPoint primerCGTGACGCGGTGCAGGGCGNeo-FCGTTGGCTACCCGTGATATT3' end of neomycin resistance gene, forward primerpENTR-FCTACAAACTCTTCCTGTTAGTTAG5' of attL1 in pENTR vector, forward primerPCDNA3.1FCTAGAGAACCCACTGCTTACRVprimer3CTAGCAAAATAGGCTGTCCC5' of MCS in pGL3 vector, forward primer35S promoterCTATCCTTCGCAAGACCCTTCCaMV 35S promoter, forward primer5'TriplEx2CTCCGAGATCTGGACGAGCEcdysone ForwardCTCTGAATACTTTCAACAAGTTACDrosophila heat shock promoter, forward primerpFastBac-3'CTGATTATGATCCTCTAGTACTTCPBV220RCTGCGTTCTGATTTAATCTGBglob-intron-FCTGGTCATCATCCTGCCTTTRabbit beta-globin intron, forward primertdTomato-FwdCTGTTCCTGTACGGCATGG3' end of tdTomato, forward primerpIRES-FCTTACTGACATCCACTTTGCPry1CTTAGCATGTCCGTGGGGTTTGAATPZ P-element, reverse primerTKpolyAreverseCTTCCGTGTTTCAGTTAGCpLXSN-FCTTGAACCTCCTCGTTCGACpBRforBamCTTGGAGCCACTATCGACIn pBR322 tet region, upstream of BamHI, forward primerpBABE 5'CTTTATCCAGCCCTCACPsi packaging signal, 5' of MCS in pBABE vectors, forward primerGLP2CTTTATGTTTTTGGCGTCTTCCASV40pA-RGAAATTTGTGATGCTATTGCSV40 polyA, reverse primerS.tagGAACGCCAGCACATGGACAUG1 ReverseGAAGAGAAAAACATTAGTTGGCFor Pichia vectors with AUG1 promoter, reverse primerLacZ-RGACAGTATCGGCCTCAGGAA5' end of LacZ, reverse primerRVP4GACGATAGTCATGCCCCGCGpbi-121(35s)GACGCACAATCCCACTATCCpMX-S1811GACGGCATCGCAGCTTGGATACACMMLV sequence, 5' of MCS in pMXs vector, forward primerHU6GACTATCATATGCTTACCGTXBG-RGACTCCATTCGGGTGTTCXenopus beta-globin 3'UTR, reverse primerTac promoterGAGCGGATAACAATTTCACACAGGTac promoter, forward primerHATGAGGAGCACGCTCATGCCCACHistidine affinity tag, forward primerpTrcHis ForwardGAGGTATATATTAATGTATCG5' of MCS in pTrcHis vector, forward primerGal4 N-termGAGTAGTAACAAAGGTCAA3' end of Gal4 DNA binding domain, forward primerpHybLex ReverseGAGTCACTTTAAAATTTGTATACACADH terminator, reverse primer3.0revGAGTTAGCTCACTCATTAGGCNOSGATAATCATCGCAAGACCGGpCMVFGATCCGGTACTAGAGGAACTGAAAAACDuetUP1GATCTCGACGCTCTCCCTMBP-FGATGAAGCCCTGAAAGACGCGCAG3' end of maltose binding protein, forward primerpYESTrp ForwardGATGTTAACGATACCAGCCDuetDOWN1GATTATGCGGCCGTGTACAApBAD Reverse|pTrcHis ReverseGATTTAATCTGTATCAGGFor vectors with E. coli araBAD promoter, reverse primerCRE-RGCAAACGGACAGAAGCATTT5' end of Cre recombinase, reverse primerPuro-FGCAACCTCCCTTCTACGAGC3' end of puromycin resistance gene, forward primerpCAG-FGCAACGTGCTGGTTATTGTGRabbit beta-globin intron, for pCAG plasmids, forward primerpCasper-hsGCAACTACTGAAATCTGCCAAGDrosophila Hsp70 promoter, forward primerCAT-RGCAACTGACTGAAATGCCTC5' end of chloramphenicol resistance gene, reverse primerNmt1-FGCAATGTGCAGCGAAACTAAS. pombe nmt1 promoter, forward primerT3GCAATTAACCCTCACTAAAGGT3 promoter, forward primerpFlag-CMV-RGCACTGGAGTGGCAACTTMycGCATCAATGCAGAAGCTGATCTCAMyc tag, forward primerGW-3'GCATGATGACCACCGATATG3' end of Gateway cassette, forward primerNeo-RGCCCAGTCATAGCCGAATAG5' end of neomycin resistance gene, reverse primerSV40-PromoterGCCCCTAACTCCGCCCATCCpbd-gal4-fGCCTCTAACATTGAGACAGCSeqL-A (ATTL1)GCGAGAGTAGGGAACTGCpCAMBIA1301-3'GCGATTAAGTTGGGTAACGCCYC1|pYESTrp ReverseGCGTGAATGTAAGCGTGACCYC1 transcription termination signal, reverse primerNOS-FGCGTTCAAAAGTCGCCTAAGNopaline synthase promoter, forward primerpAd-CMVGCTAGAGATCTGGTACCGTCFor cloning sites after SalI in pAd-CMV vectorT7 TerminalGCTAGTTATTGCTCAGCGGT7 terminator, reverse primerpREP ForwardGCTCGATACAATAAACGCCRous sarcoma virus (RSV) promoter, forward primerMT1-FGCTGTCCTCTAAGCGTCACCMouse metallothionein 1 promoter, forward primerpBMN 5'GCTTGGATACACGCCGCMMLV sequence, for inserts in pBMN retroviral vectorM13 Reverse (-27)GGAAACAGCTATGACCATGSV40-FGGAGGCTTTTTTGGAGGC3'AOXGGCAAATGGCATTCTGACATPCDNA3.0-R2GGCAACTAGAAGGCACAGTCLacI-RGGCATACTCTGCGACATCGT5' end of LacI, reverse primerTet-RGGCGAGTTTACGGGTTGTTA5' end of tetracycline resistance gene, reverse primerpTarget.RGGCTTTACACTTTATGCTTCpBR322ori-FGGGAAACGCCTGGTATCTTTpBRS322 origin, forward primerU6 PrimerGGGCAGGAAGAGGGCCTATpGEX 5'GGGCTGGCAAGCCACGTTTGGTG3' end of glutathione-S-transferase, forward primerTn7-endGGGGTGGAAATGGAGTTTTTBacterial transposon Tn7pCasper-FGGGTTTTATTAACTTACAT5' end of Drosophila mini-white gene, reverse primerpFlag-CMV-FGGTAGGCGTGTACGGTGGGFP-FGGTCCTTCTTGAGTTTGTAAC3' end of GFP, forward primerMalE PrimerGGTCGTCAGACTGTCGATGAAGCCpSHUTTLE-CMV-FGGTCTATATAAGCAGAGGTGpBRrevBamGGTGATGTCGGCGATATAGGIn pBR322 tet region, downstream of BamHI, reverse primerGal10pro-FGGTGGTAATGCCATGTAATATGS. cerevisiae GAL10 promoter, forward primer1492RGGTTACCTTGTTACGACTTM13 Forward (-20)GTAAAACGACGGCCAGTGS6GTAAATGAATTTTCTGTAGTAGGpPc86-3'GTAAATTTCTGGCAAGGTAGACDsRed1-NGTACTGGAACTGGGGGGACAG5' end of DsRed1, reverse primerPIRES2-EGFP-FGTAGGCGTGTACGGTGGGAGPolyhedrin reverseGTCCAAGTTTCCCTGFor baculovirus vector with polyhedrin promoter, reverse primerpFastBac-5'GTCCGAAACCATGTCGTACEXFP-RGTCTTGTAGTTGCCGTCGTCFor distinguishing EGFP vs ECFP vs EYFP, reverse primerpACT2-RGTGCACGATGCACAGTTGAAF1ori-FGTGGACTCTTGTTCCAAACTGGF1 origin, forward primerpSHUTTLE-CMV-RGTGGTATGGCTGATTATGATCAGEBV ReverseGTGGTTTGTCCAAACTCATCSV40 polyA terminator, reverse primerCMV-RGTTCACGGTGCCCTCCpQE30-RGTTCTGAGGTCATTACTGGpcDL-FGTTGCCTTTACTTCTAGGCCT5' of EcoRI site in pcDL vector, forward primerpLXSN-RGTTGCTGACTAATTGAGATGM13 (-40)GTTTTCCCAGTCACGACIn lacZ genepLEXA-RTAAAACCTAAGAGTCACTTT3'BDTAAGAGTCACTTTAAAATTTGTAT7TAATACGACTCACTATAGGGT7 promoter, forward primerGAL4 ADTACCACTACAATGGATGHA-FTACCCATACGACGTCCCAGAHA tag, forward primerSP6TACGATTTAGGTGACACTATAGα-FactorTACTATTGCCAGCATTGCTGCBGH Reverse|pcDNA3.1RTAGAAGGCACAGTCGAGGBovine growth hormone terminator, reverse primerpPc86FTATAACGCGTTTGGAATCACTXpress ForwardTATGGCTAGCATGACTGGTXpress epitope, forward primerPEGFP-CTATGGCTGATTATGATCAGTSV40pro-FTATTTATGCAGAGGCCGAGGSV40 promoter/origin, forward primerEF-1a ForwardTCAAGCCTCAGACAGTGGTTCHuman elongation factor-1a promoter, forward primerGAL4 BDTCATCGGAAGAGAGTAGGAL4-BDforTCATCGGAAGAGAGTAGTAApCMV-RTCCAAACTCATCAATGTATCpDsRED-express-C1-FTCCCACAACGAGGACTACAChrGFP-RTCCCCGAGTACCACTTCATChrGFP (humanized Renilla GFP), forward primerITS1TCCGTAGGTGAACCTGCGGITS4TCCTCCGCTTATTGATATGCpZIPTCCTTTCCAGCGAGGTTCTAMurine leukemia virus (MuLV), reverse primerpBluescriptKSTCGAGGTCGACGGTATCFor pBluescript vectorH1TCGCTATGTGTTCTGGGAAAHuman H1 promoter, forward primerpYes2.RTCGGTTAGAGCGGATGTGpCMV-FTCTAAAAGCTGCGGAATTGTpBluescriptSKTCTAGAACTAGTGGATCFor pBluescript vectorHA-RTCTGGGACGTCGTATGGGTAHA tag, reverse primertdTomato-RevTCTTTGATGACGGCCATGT5' end of tdTomato, reverse primerpDSRED-N1-RTGAAGCGCATGAACTCCTTGhUBCpro-FTGAAGCTCCGGTTTTGAACTHuman Ubiquitin C (UbC) promoter, forward primerpACYC-FTGAAGTCAGCCCCATACGATp15A origin, forward primerpQE30-FTGAGCGGATAACAATTTCACP5'|pMAL-C2X-FTGCGTACTGCGGTGATCAACT7TERTGCTAGTTATTGCTCAGCGGIRES-FTGGCTCTCCTCAAGCGTATT3' end of IRES, forward primerpEGFP-N-5’TGGGAGGTCTATATAAGCAGAGM13 (-21) ForwardTGTAAAACGACGGCCAGTIn lacZ geneGLP1TGTATCTTATGGTACTGTAACTGC-CMV-24TTAGGACAAGGCTGGTGGPCMV-5FTTCCAAAATGTCGTAATAACpMT2-FTTGCCTTTCTCTCCACAGGT3' end of synthetic intron, forward primerpTRC99C-FTTGCGCCGACATCATAACmCherry-RTTGGTCACCTTCAGCTTGG5' end of mCherry, reverse primerDuetUP2TTGTACACGGCCGCATAATCTK-pA-RTTGTCTCCTTCCGTGTTTCAThymidine kinase polyA, reverse primerXEF1aTTTCGCCCTAACTTCGTGATXenopus EF1 alpha enhancer/promoter, forward primerBglob-intron-RTTTGCCCCCTCCATATAACARabbit beta-globin intron, reverse primerP12584TTTTCAGTATCTACGATBglob-pA-RTTTTGGCAGAGGGAAAAAGARabbit beta-globin polyA region, reverse primer
Souce: 纽普生物 2017-03-01
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