Human coagulation factor VII is a single chain glycoprotein that is vitamin K-dependent and plays an extremely important role in the process of coagulation,which has been widely used in the clinic. It can be used for hemostatic purposes such as hemophilia with inhibitors,congenital FVII deficiency, thrombocytopenia and traumatic bleeding caused by surgery or severe trauma. The gene recombination technology is an attractive methed which provides more effective ways to produce recombinant human coagulation factor VII on a large scale. In recent years, a variety of recombinant expression systems of human coagulation factor VII have been tried and established. In this paper, it is focused on the development and application of recombinant human coagulation factor VII in the expression systems of yeasts, mammalian cells and transgenic animals and compares the characteristics of post-translational modification of different mammalian cells, so as to provide reference for the further development of recombinant human coagulation factor VII in the recombinant expression system.