Glutamine Assay kit (Colorimetric) ab197011 is a simple and sensitive assay that detects the biologically relevant concentrations of glutamine in biological fluids and tissues.

The glutamine assay protocol is based on the hydrolysis of Glutamine to Glutamate producing a stable colored signal, which is directly proportional to the amount of glutamine in the sample.

The assay can detect as little as 25 μM of glutamine in a variety of biological samples.

Glutamine assay protocol summary:- add samples and standards to wells- add hydrolysis mix and incubate for 30 min- add reaction mix and incubate for 60 min- analyze with a microplate reader.