在这项研究中，已研究了双偶氮染料分子家族（BA1-BA5）作为通用化合物。确定并讨论了胺部分中各种连接基团对化合物的热稳定性和电化学行为的影响。已经发现具有脂族基二胺部分的BA1和BA2比其他的具有更高的热稳定性。此外，还研究了BA1-BA5在沸水，酸和碱性介质中的化学稳定性。所有化合物均显示出对沸水的强抵抗力。此外，BA3-BA5用6N HCl处理后，含芳族二胺部分的含氟化合物稳定。此功能可能使它们适合用作光学记录设备中的染料介质。Ag纳米颗粒以绿色方式与各种双偶氮染料分子反应生成AgNPs / BA1-BA5杂化物。使用原子吸收光谱仪（AAS）确定每个杂化物中的Ag含量。双偶氮化合物及其纳米银对应物的抗菌活性已在体外针对各种病原体进行了筛选，这些病原体包括耐甲氧西林的金黄色葡萄球菌（MRSA），粪肠球菌（E. faecalis），伤寒沙门氏菌（S.typhi），铜绿假单胞菌（P. aeruginosa）），大肠杆菌（E. coli），金黄色葡萄球菌（S. aureus））和曲霉菌。与双偶氮化合物相反，AgNPs / BA杂种对革兰氏阳性和阴性细菌均有效。此外，AgNPs / BA3和AgNPs / BA5能够有效抑制曲霉菌的生长。

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In this study, a family of bisazo dye molecules (BA1-BA5) has been investigated as versatile compounds. Influence of various linking groups in amine moiety on thermal stability and electrochemical behavior of the compounds was determined and discussed. BA1 and BA2 with aliphatic-based diamine moiety have been found to be more thermal stable than others. Besides, the chemical stability of BA1-BA5 was studied in boiling water, acid and base media. All compounds showed strong resistance toward boiling water. Moreover, BA3-BA5 containing aromatic diamine moiety were stable after treatment with 6 N HCl. This feature may make them suitable as dye media in optical recording devices. Ag nanoparticles reacted with various bisazo dye molecules in a green approach to produce AgNPs/BA1-BA5 hybrids. Ag content in each hybrid was determined using an atomic absorption spectrometer (AAS). Antimicrobial activity of bisazo compounds and their nano silver counterparts has been screened in vitro against various pathogens including Methicillin-resistant Staphylococcus aureus (MRSA), Enterococcus faecalis (E. faecalis), Salmonella typhi (S.typhi), Pseudomonas aeruginosa (P. aeruginosa), Escherichia coli (E. coli), Staphylococcus aureus (S. aureus) and Aspergillus fungi. Contrary to the bisazo compounds, AgNPs/BA hybrids were effective on both Gram-positive and negative bacteria. Moreover, AgNPs/BA3 and AgNPs/BA5 were able to efficaciously inhibit the growth of Aspergillus fungus.