哺乳动物的肝脏具有小叶结构，门脉三联体由门静脉、肝动脉和胆管组成，表现出带状基因表达，而硬骨鱼则没有门脉三联征。它们具有什么样的单元结构，包括它们的基因表达模式，还有待证明。本研究的目的是展示硬骨鱼肝脏的单位结构，并就脊椎动物的进化和适应以及使用硬骨鱼作为人类疾病的替代模型进行讨论。斑马鱼肝脏在其形态结构和基因表达方面作为硬骨鱼的代表进行了检查。在斑马鱼肝脏中检测到一种新颖的多边形小叶结构。其中门静脉和中央静脉分别分布在外围和中心。正弦曲线连接两条静脉。Anxa4 阳性前导管被纳入切片中两排肝细胞的管腔中。肝内胆管随机分布在肝小叶中。斑马鱼肝脏没有检测到代谢途径的带状基因表达。斑马鱼肝脏的小叶与位于肝细胞管腔中的前导管可能类似于哺乳动物受损肝脏的卵圆细胞反应，并且可能比哺乳动物的肝脏更安全地将胆汁输送到肠道。肝小叶中的基因表达模式和我们的斑马鱼肝小叶模型对于讨论使用这种动物作为人类疾病替代模型的实验中获得的数据可能很重要。Anxa4 阳性前导管被纳入切片中两排肝细胞的管腔中。肝内胆管随机分布在肝小叶中。斑马鱼肝脏没有检测到代谢途径的带状基因表达。斑马鱼肝脏的小叶与位于肝细胞管腔中的前导管可能类似于哺乳动物受损肝脏的卵圆细胞反应，并且可能比哺乳动物的肝脏更安全地将胆汁输送到肠道。肝小叶中的基因表达模式和我们的斑马鱼肝小叶模型对于讨论使用这种动物作为人类疾病替代模型的实验中获得的数据可能很重要。Anxa4 阳性前导管被纳入切片中两排肝细胞的管腔中。肝内胆管随机分布在肝小叶中。斑马鱼肝脏没有检测到代谢途径的带状基因表达。斑马鱼肝脏的小叶与位于肝细胞管腔中的前导管可能类似于哺乳动物受损肝脏的卵圆细胞反应，并且可能比哺乳动物的肝脏更安全地将胆汁输送到肠道。肝小叶中的基因表达模式和我们的斑马鱼肝小叶模型对于讨论使用这种动物作为人类疾病替代模型的实验中获得的数据可能很重要。斑马鱼肝脏的小叶与位于肝细胞管腔中的前导管可能类似于哺乳动物受损肝脏的卵圆细胞反应，并且可能比哺乳动物的肝脏更安全地将胆汁输送到肠道。肝小叶中的基因表达模式和我们的斑马鱼肝小叶模型对于讨论使用这种动物作为人类疾病替代模型的实验中获得的数据可能很重要。斑马鱼肝脏的小叶与位于肝细胞管腔中的前导管可能类似于哺乳动物受损肝脏的卵圆细胞反应，并且可能比哺乳动物的肝脏更安全地将胆汁输送到肠道。肝小叶中的基因表达模式和我们的斑马鱼肝小叶模型对于讨论使用这种动物作为人类疾病替代模型的实验中获得的数据可能很重要。

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The mammalian liver has a lobule structure with a portal triad consisting of the portal vein, hepatic artery, and bile duct, which exhibits zonal gene expression, whereas those of teleosts do not have a portal triad. It remains to be demonstrated what kind of the unit structures they have, including their gene expression patterns. The aims of the present study were to demonstrate the unit structure of the teleost liver and discuss it in terms of evolution and adaptation in vertebrates and the use of teleosts as an alternative model for human disease. The zebrafish liver was examined as a representative of teleosts with respect to its morphological architecture and gene expression. A novel, polygonal lobule structure was detected in the zebrafish liver. In it, portal veins and central veins were distributed at the periphery and center, respectively. Sinusoids connected both veins. Anxa4-positive preductules were incorporated into the tubular lumen of two rows of hepatocytes in sections. Intrahepatic bile ducts resided randomly in the liver lobule. Zebrafish livers did not have zonal gene expression for metabolic pathways examined. The lobules of the zebrafish liver with preductules located in the tubular lumina of hepatocytes may resemble the oval cell reaction of injured livers of mammals and might convey bile to the intestine more safely than mammalian livers. The gene expression pattern in liver lobules and our liver lobule model of the zebrafish may be important to discuss data obtained in experiments using this animal as an alternative model for human disease.