菌菇是一种食用和药用的产菌核蘑菇。这种蘑菇的菌核也可用作食品和民间药物。基于对其单核基因组的描述，采用Illumina和PacBio测序技术进行测序，并利用RNA测序（RNA-seq）进行比较转录组分析，研究其菌核形成机制。这从头组装后的基因组大小为 35.82 Mb，N50 支架大小为 4.29 Mb，编码 12,173 个推定蛋白质。表达分析表明，随着菌核的形成，分别有 1,146 个和 1,249 个基因上调和下调。差异表达基因与底物分解、氧化还原过程、细胞壁合成和其他生物过程有关。块茎马铃薯。这些基因组和转录组资源为菌核形成的机制提供了有用的信息。块茎马铃薯。

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Pleurotus tuber-regium is an edible and medicinal sclerotium-producing mushroom. The sclerotia of this mushroom also serve as food and folk medicine. Based on the description of its monokaryon genome, sequenced with Illumina and PacBio sequencing technologies, comparative transcriptomic analysis using RNA sequencing (RNA-seq) was employed to study its mechanism of sclerotium formation. The de novo assembled genome is 35.82 Mb in size with a N50 scaffold size of 4.29 Mb and encodes 12,173 putative proteins. Expression analysis demonstrated that 1,146 and 1,249 genes were upregulated and downregulated with the formation of sclerotia, respectively. The differentially expressed genes were associated with substrate decomposition, the oxidation-reduction process, cell wall synthesis, and other biological processes in P. tuber-regium. These genomic and transcriptomic resources provide useful information for the mechanism underlying sclerotium formation in P. tuber-regium.