在本研究中，研究了海带多糖（LJPA-P）的结构特征和抗氧化活性。从海带中获得了三种酸性多糖LJPA-P1，LJPA-P2和LJPA-P3依次用热水和柠檬酸萃取，然后用DEAE-Sepharose快速流色谱和连续的NaCl溶液洗脱进行分馏。结果显示三种酸性多糖的单糖组成，硫酸化作用，糖苷键，分子量和抗氧化活性均不同。LJPA-P3的独特之处在于其高硫酸盐基团和半乳糖含量，半乳糖，甘露糖和岩藻糖的摩尔比（26.1：1.3：1）和1,3-和1-连接半乳糖的优势，显示出很高的氧自由基吸收能力（ ORAC，1247.22μmolTrolox / g）和ABTS自由基清除活性（高达70％），与LJPA-P1和LJPA-P2完全不同。因此，褐紫菜海带 代表具有重要抗氧化活性的多糖的良好来源。

"点击查看英文标题和摘要"

In the present study, polysaccharides from Laminaria japonica (LJPA-P) were investigated for their structural characteristics and antioxidant activity. Three acidic polysaccharides LJPA-P1, LJPA-P2 and LJPA-P3 were obtained from Laminaria japonica by sequential hot water and citric acid extraction, and fractionation by DEAE-Sepharose fast flow chromatography and serial NaCl solution elutions. Results showed that three acidic polysaccharides differed in their monosaccharide compositions, sulfation, glycosidic linkages, molecular weights and antioxidant activities. LJPA-P3 was unique in its high sulfate group and galactose contents, molar ratio of galactose, mannose and fucose (26.1: 1.3: 1) and dominance of 1,3- and 1-linked galactose, exhibiting remarkably high oxygen radical absorbance capacity (ORAC, 1247.22 μmol Trolox/g) and ABTS radical scavenging activity (up to 70%), which was quite different from those of LJPA-P1 and LJPA-P2. Therefore, brown seaweed Laminaria japonica represents a good source of polysaccharides with significant antioxidant activity.