pFastBac Dual 载体图谱和序列 |
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载体基本信息
载体名称
pFastBac Dual
载体抗性
Ampicillin
载体长度
5238 bp
载体类型
Insect Cell Vectors
载体来源
Invitrogen (Life Technologies)
拷贝数
High copy number
pFastBac Dual 载体载体图谱
pFastBac Dual5238 bp6001200180024003000360042004800f1 oriAmpR promoterAmpRoriTn7RGmRPc promoterHSV TK poly(A) signalMCSp10 promoterpolyhedrin promoterMCSSV40 poly(A) signalTn7L
pFastBac Dual 载体载体序列
Copy Sequence
下载GeneBank文件(.gb) LOCUS Exported 5238 bp ds-DNA circular SYN 04-4-2014 DEFINITION Dual-promoter vector for co-expression of two proteins in insect cells using the Bac-to-Bac(R) baculovirus system. ACCESSION . VERSION . KEYWORDS pFastBac Dual SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5238) AUTHORS Invitrogen (Life Technologies) TITLE Direct Submission COMMENT Transform into DH10Bac(TM) E. coli cells. FEATURES Location/Qualifiers source 1..5238 /organism="synthetic DNA construct" /lab_host="Insect Cells" /mol_type="other DNA" rep_origin 102..557 /direction=RIGHT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 584..688 /gene="bla" /note="AmpR promoter" CDS 689..1549 /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 1720..2308 /direction=RIGHT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" mobile_element 2611..2835 /mobile_element_type="transposon:Tn7" /note="Tn7R" /note="mini-Tn7 element (right end of the Tn7 transposon)" CDS complement(2902..3435) /codon_start=1 /gene="aacC1" /allele=" " /product="gentamycin acetyltransferase" /note="GmR" /note="confers resistance to gentamycin" /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR EEVMHFDIDPSTAT" promoter complement(3624..3652) /gene="intI1 (promoter lies within the coding sequence)" /note="Pc promoter" /note="class 1 integron promoter" polyA_signal 4161..4209 /note="HSV TK poly(A) signal" /note="herpesvirus thymidine kinase polyadenylation signal" misc_feature 4274..4337 /note="MCS" /note="multiple cloning site" promoter complement(4345..4454) /note="p10 promoter" /note="baculovirus promoter for expression in insect cells" promoter 4478..4569 /gene="polh from Autographa californica" /note="polyhedrin promoter" /note="promoter for the baculovirus polyhedrin gene" misc_feature 4606..4704 /note="MCS" /note="multiple cloning site" polyA_signal 4828..4962 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" mobile_element 4991..5156 /mobile_element_type="transposon:Tn7" /note="Tn7L" /note="mini-Tn7 element (left end of the Tn7 transposon)"我们不提供该载体，载体信息仅供查询和参考。 |
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