Antibody Purification Using Protein A and Alkaline Stable Protein A |
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Antibody purification involves isolation of antibody from serum (polyclonal antibody), ascites fluid, or from the culture supernatant of a hybridoma cell line (monoclonal antibody). Protein A ligand is recommended specifically for polyclonal IgG from rabbit, pig, dog, or cat serum. It binds the heavy chain constant region (Fc) of IgG (CH2-CH3 region). Native Protein A is purified from Staphylococcus aureus (46.7 kDa; 4 IgG-binding sites). The POROS MabCapture A resins feature a recombinant Protein A (rPA) ligand produced in yeast that can be scaled up for bioprocess applications. The MabCaptureC™ High Capacity Protein A is a high-performance chromatography resin for capturing mAbs and recombinant Fc-tagged fusion proteins. It provides high specific binding capacity and enhanced alkaline stability characteristics under clean-in-place conditions (CIP), for a more robust purification and resin regeneration. We offer a wide selection of base supports and formats to purify antibodies using the Protein A ligand. Our portfolio is designed to meet small-scale (screening) up to large-scale (bioprocess) needs. Multiple formats—magnetic beads, loose resin, spin columns and kits, FPLC cartridges, and 96-well filter plates enable antibody purification from microgram to kilogram scalesAlkaline stability—engineered to withstand common CIP procedures with 0.2 M NaOH enabling efficient cleaning of the chromatography resin High performance—resins are designed to maximize protein yield and reduce backgroundEconomical—pricing is similar to or better than our leading competitors Choose the right Protein A product for antibody purificationProduct links in the last row of each column Dynabeads Protein A Magnetic BeadsPierce Protein A AgarosePierce Protein A Plus AgarosePierce Recombinant Protein A AgaroseMabCaptureC™ High Capacity Protein A, Alkaline-StableBead or resin size2.8 μm45–165 μm45–165 μm45–165 μm75 μmBinding capacity250–300 μg human IgG/mL beads12–19 mg human IgG/mL resin34 mg human IgG/mL resin15–17 mg human IgG/mL resin58 mg/ml at 5 min residence (80 mg/ml at 10 min residence) – 10% breakthroughMaximum linear flow rateN/A700 cm/hr700 cm/hr700 cm/hr300 cm/hr or less (150 cm/hr recommended)SupportMonosized, super paramagnetic polystyrene beadsCrosslinked 6% beaded agaroseCrosslinked 6% beaded agaroseCrosslinked 6% beaded agaroseHighly cross-linked agaroseRecommended application scalescreeningbatch, pilotscreening, batchbatchbatch, pilot, processFormats availableloose beadsloose beads, chromatography cartridgesloose beads, pre-packed spin columns and kits, 96-well filter platesloose beadsloose beadsOrder product(s)10001D20333 (loose resin)88924 (1 mL cartridge)89925 (5 mL cartridge)44667 (spin kit)22810 (loose resin)45202 (spin plates)20365A53031, 10 mL 50% slurryA53033, 50 mL 50% slurryA53034, 200 mL 50% slurry1963662250, 250 mL 50% slurry196366201L, 1 L 50% slurry196366205L, 5 L 50% slurry 196366210L, 10 L 50% slurry89952,89956,89960,89948,89978,21001,21004,21020, 21027,21030 资源 抗体纯化学习资源蛋白质生物学学习中心抗体学习中心抗体制备和纯化介绍抗体纯化方法抗体IgG结合蛋白比较支持资源NEW 抗体和免疫检测支持中心 |
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