Thank you for contacting us.

Unfortunately we have not determined the exact concentration for this product. Before conjugation with FITC this product has a 1mg/ml concentration. When using isotype controls in Flow Cytometry keep in mind that it is recommended that concentrations are really antibody amount in excess. Therefore we would recommend using ab18435 Mouse IgG1 Isotype control at a concentration matching or exceeding 1mg/ml.

Abcam also carries other products essential for Flow Cytometry such as isotype controls. If testing live cells we also recommend cell viability controls such as 7-AAD or Propidium Iodide. Propidium Iodide (PI) binds to double-stranded DNA. PI cannot cross intact plasma membrane and therefore will only be present in DNA of cells where the plasma membrane has been compromised/ permeabilized. We do carry PI and offer a 7-AAD Cell-Mediated Cytotoxicity Assay Kit.

Propidium Iodide: https://www.abcam.com/Propidium-Iodide-ab14083.html

7-AAD Kit: https://www.abcam.com/7-AADCFSE-Cytotoxicity-Assay-Kit-ab133073.html

I hope this information has been of help to you. If you have any further questions, please do not hesitate to ask.

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回复于 Oct 25 2012