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GM508 CultActive
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GM508 CultActive
Bicarbonate buffered
Ready to use
For investigation of fertilization failure (deficient oocyte activation)
Contains Ca2+-Ionophore A23187
A Certificate of Analysis for each batch is available at hamiltonthorne.com
GM508 CultActive Intended Use
GM508 CultActive is a bicarbonate buffered, HSA free reagent designed to investigate oocytes of patients with failed fertilization after previous intracytoplasmatic sperm injection (ICSI) cycles. GM508 CultActive is designed to investigate if fertilization failure after previous ICSI cycles is due to a deficient oocyte activation. *GM508 CultActive is for non-clinical, research use only in USA. GM508 CultActive Instructions for Use Prior to use, both GM508 CultActive and culture media (i.e.,GM501 Cult) must be equilibrated for 4 hours in vial not firmly closed at 5 - 7% CO₂ and 37°C prior to use. Any laboratory procedures described herein are recommendations only. Each laboratory must establish and validate its own procedures for preparation and use. See below for recommended application. GM508 CultActive Composition NaCl, KCl, KH2PO4, MgSO4 CaCl2 Bicarbonate, EDTA Glucose, Lactate, Pyruvate Non-essential and essential Amino Acids, Alanyl-Glutamine Ca2+-Ionophore A23187, DMSO GM508 CultActive Specifications pH (at 37°C, 6%CO2) 7.00 - 7.60 Osmolality (mOsm/kg) 400 - 460 Sterility (sterile/SAL 10-3) No Growth Endotoxins (LAL, EU/ml) < 0.25 1-cell MEA ≥ 80% blastocysts at 96h after 1h exposure to GM508 CultActive GM508 CultActive Order Codes Part # Size Storage Shelf Life* 4 GM 508CULT-active1 1 x 1 ml 2 - 8°C6 months * From date of manufacture CultActive ReferencesMurugesu S, Saso S, Jones BP, et al. Does the use of calcium ionophore during artificial oocyte activation demonstrate an effect on pregnancy rate? A meta-analysis. Fertil Steril. 2017;108(3):468-482.e3. doi:10.1016/j.fertnstert.2017.06.029 Ebner T, Montag M; Oocyte Activation Study Group, et al. Live birth after artificial oocyte activation using a ready-to-use ionophore: a prospective multicentre study. Reprod Biomed Online. 2015;30(4):359-365. doi:10.1016/j.rbmo.2014.11.012 MKT-2015 Rev. A, 06-Nov-2020 Attachments: FileDescriptionFile size MKT-2015 GM508 CultActive Rev.AGM508 CultActive Brochure 1666 kB GM508 CultActive GYN IFU.pdfGM508 CultActive Instructions for Use3401 kB |
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