秦慧民，博士，天津科技大学生物工程学院教授，博士生导师。天津市高层次人才，2013年于东京大学获得博士学位，2013-2015年在东京大学从事博士后研究。获得日本文部科学省国费奖学金，东京大学农学生命科学研究科科长受赏。2015年6月入职天津科技大学，2017年晋升教授，中国微生物学会会员。致力于工业酶制剂的结构与功能解析、酶工程领域的研究。在Angewandte Chemie International Edition, ACS Catalysis, Bioresource Technology, Food Chemistry等国际知名杂志发表论文30余篇，获授权专利6项，主持国家重点研发、国家自然科学基金、天津市应用基础与前沿技术研究计划等项目。担任Frontiers in Bioengineering and Biotechnology，Food等杂志客座编辑。

学习经历：

2010.4-2013.3 东京大学应用生命化学专业　博士

2004.9-2008.7 华南理工大学制糖工程专业 硕士

2000.9-2004.7 山东农业大学食品科学与工程专业 本科

工作经历：

2017/12-至今 天津科技大学, 教授; 主要研究方向: 发酵工程;酶工程

2015/6-2017/11 天津科技大学, 讲师; 主要研究方向: 发酵工程;酶工程

2013/4-2015/5 东京大学应用生命化学专业 特任助教

2008/10-2009/8 东北师范大学中国赴日本国留学生预备学校 日语学习

主讲课程：

本科生：微生物学实验，微生物遗传学实验

研究生：结构生物学

留学生：Microbial Genetics and Breeding Experiment; Protein Structure Simulation and Design

科研领域及方向

[1] 功能糖生物转化研究与开发；

[2]酶制剂的结构与功能解析；

[3] 酶的理性设计与定向进化；

[4]工业酶/菌株的高通量筛选

科研情况

[1]国家重点研发计划，绿色工业酶的高通量筛选与智能设计改造，2022.11-2025.10，在研，主持项目

[2]国家自然科学基金面上项目，维生素D3前体生物合成关键酶胆固醇7-脱氢酶系的构效研究，2018.1-2021.12，结题，主持项目

[3]天津市应用基础与前沿技术研究计划，稀少糖转化酶D-阿洛酮糖3-差向异构酶的高通量筛选与定向改造，2019.11-2022.10, 结题，主持项目

[4]天津市自然科学基金青年项目，海藻酸多糖降解酶的结构生物学与功能研究，2016.4-2019.3， 结题，主持项目

主要学术成果

近期代表作

2023

1) Li, C.; Gao, X.; Qi, H.; Zhang, W.; Li, L.; Wei, C.; Wei, M.; Sun, X.; Wang, S.; Wang, L.; Ji, Y.; Mao, S.; Zhu, Z.; Tanokura, M.; Lu, F.; Qin, H.-M.* Substantial improvement of an epimerase for the synthesis of D-allulose by biosensor-based high-throughput microdroplet screening. Angew. Chem. Int. Ed. 2023, e202216721

2022

1) Gao, X.; Wei, C.; Qi, H.; Li, C.; Lu, F.; Qin, H.-M.* Directional immobilization of D-allulose 3-epimerase using SpyTag/SpyCatcher strategy as a robust biocatalyst for synthesizing D-allulose. Food Chem. 2023, 401, 134199

2) Zhang, W.; Wei, M.; Sun X.; Lu, F.; Guan, L.; Mao S.;* Qin, H.-M.* Fine-tuning of carbon flux and artificial promoters in Bacillus subtilis enables high-level biosynthesis of D‑allulose. J. Agric. Food Chem. 2022, 70, 13935−13944

3) Li, L.; Zhang, Q.; Wang T.; Qi, H.; Wei, M.; Lu, F.; Guan, L.; Mao S.;* Qin, H.-M.* Engineering of acid-resistant D-allulose 3-epimerase for functional juice production. J. Agric. Food Chem. 2022, 70, 16298−16306

2021

1) Li, C., Li, L., Feng, Z., Guan, L., Lu, F.*, Qin, H.-M.* Two-step biosynthesis of D-allulose via a multienzyme cascade for the bioconversion of fruit juices. Food Chem. 2021, 357, 129746

2) Li, C., Zhang, W., Wei, C., Gao, X., Mao, S.*, Lu, F.*, Qin, H.-M.* Continuous spectrophotometric assay for high-throughput screening of predominant D-allulose 3-epimerases. J. Agric. Food Chem. 2021, 69: 11637-11645

3) Gao, X., Wu, M., Zhang, W., Li, C., Guo, R.-T., Dai, Y., Liu, W., Mao, S.*, Lu, F.*, Qin, H.-M.* Structural basis of salicylic acid decarboxylase reveals a unique substrate recognition mode and access channel. J. Agric. Food Chem. 2021, 69: 11616-11625

4) Mao, S., Chen, Y., Sun, J., Wei, C., Song, Z., Lu, F.*, Qin, H.-M.* Enhancing the sustainability of KsdD as a biocatalyst for steroid transformation by immobilization on epoxy support. Enzyme Microb. Technol. 2021, 146: 109777

5) Zhu, Z., Li, L., Zhang, W., Li, C., Mao, S.*, Lu, F.*, Qin, H.-M.* Improving the enzyme property of D-allulose 3-epimerase from a thermophilic organism of Halanaerobium congolense through rational design. Enzyme Microb. Technol. 2021, 149: 109850

2020

1) Wang, F., Zhu, M., Song, Z., Zhu, Z., Lu, F.,* Qin, H.-M.* Reshaping the binding pocket of lysine hydroxylase for enhanced activity.ACS Catal. 2020, 10: 13946-13956

2) Zhu, Z., Gao, X., Song, Z., Li, C., Lu, F.*, Tanokura, M.*, Qin, H.-M.* Development of engineered ferredoxin reductase systems for the efficient hydroxylation of steroidal substrates. ACS Sustain. Chem. Eng. 2020, 8: 16720-16730;

3) Mao, S., Liu, X., Gao, X., Zhu, Z., Sun, D., Lu, F.*, Qin, H.-M.* Design of an efficient whole-cell biocatalyst for the production of hydroxyarginine based on a multi-enzyme cascade. Bioresource Technol. 2020, 318, 124261

4) Li, C., Wu, M., Gao, X., Zhu, Z., Li, Y., Lu, F.*, Qin, H.-M.* Efficient biosynthesis of 2'-fucosyllactose using an in vitro multienzyme cascade. J. Agric. Food Chem. 2020, 68: 10763-10771

5) Qin, H.-M., Gao, D., Zhu, M., Li, C., Zhu, Z., Wang, H., Liu, W.*, Tanokura, M.*, Lu, F.* Biochemical characterization and structural analysis of ulvan lyase from marine Alteromonas sp. reveals the basis for its salt tolerance. Int. J. Biol. Macromol. 2020,147, 1309-1317

6) Mao, S., Song, Z., Wu, M., Wang, X., Lu, F.*, Qin, H.-M.* Expression, purification, refolding and characterization of a neverland protein from Caenorhabditis elegans. Front. Bioeng. Biotechnol. 2020, doi: 10.3389/fbioe.2020.593041

7) Mao, S., Cheng, X., Zhu, Z., Chen, Y., Li, C., Zhu, M., Liu, X., Lu, F.*, Qin, H.-M.* Engineering a thermostable version of D-allulose 3-epimerase from Rhodopirellula baltica via site-directed mutagenesis based on B-factors analysis. Enzyme Microb. Technol. 2020, 132: 109441

2019

1) Sun, D., Gao, D., Liu, X., Zhu, M., Li, C., Chen, Y., Zhu, Z., Lu, F.*, Qin, H.-M.* Redesign and engineering of a dioxygenase targeting biocatalytic synthesis of 5-hydroxyl leucine. Catal. Sci & Technol. 2019, 9: 1825-1834

2) Sun, D., Liu, X., Zhu, M., Chen, Y., Li, C., Cheng, X., Zhu, Z., Lu, F.*, Qin, H.-M.* Efficient biosynthesis of high-value succinic acid and 5-hydroxyleucine using a multienzyme cascade and whole-cell catalysis. J. Agric. Food Chem. 2019,67, 12502-12510

3) Zhu, Z., Gao, D., Li, C., Chen, Y., Zhu, M., Liu, X., Tanokura, M.*, Qin, H.-M.*, Lu, F. Redesign of a novel D-allulose 3-epimerase from Staphylococcus aureus for thermostability and efficient biocatalytic production of D-allulose. Microb. Cell Fact. 2019, 18:59

4) Mao, S., Guo, Q., Xu, P., Gao, D., Cheng, X., Zhu, M., Sun, D., Zhu, Z., Lu F.,* Qin, H.-M.* Biochemical and structural characterization of 3-ketosteroid-∆1-dehydrogenase, a candidate enzyme for efficient bioconversion of steroids. J. Chem. Technol. Biotechnol. 2019, 94: 309-316

5) Zhu, Z., Li, C., Liu, X., Gao, D., Wang, X., Tanokura, M.*, Qin, H.-M.*, and Lu, F.* Biochemical characterization and biocatalytic application of a novel D-tagatose 3-epimerase from Sinorhizobium sp. RSC Adv. 2019, 9: 2919-2927

6) Mao, S., Liu, Y., Yang, J. Ma, X., Zeng, F., Zhang, Z., Wang, S., Han, H., Qin, H.-M.*, Lu, F.* Cloning, expression and characterization of a novel fructosyltransferase from Aspergillus niger and its application in the synthesis of fructooligosaccharides. RSC Adv. 2019, 9: 23856-23863

7) Zhu, Z., Li, C., Cheng, X., Chen, Y., Zhu, M., Liu, X., Mao, S., Qin, H.-M.*, Lu, F.* Soluble expression, purification and biochemical characterization of a C-7 cholesterol dehydrogenase from Drosophila melanogaster. Steroids 2019, 152, 108495

2018年

1) Qin, H.-M., Miyakawa, T., Inoue, A., Nishiyama, R., Nakamura, A., Asano, A., Ojima, T., Tanokura, M. Structural basis for controlling the enzymatic properties of polymannuronate preferred alginate lyase FlAlyA from the PL-7 family. Chem. Commun. 2018, 54: 555-558

2) Mao, S., Wang, J.-W., Liu, F., Zhu, Z., Gao, D., Guo, Q., Xu, P., Ma, Z., Hou, Y., Cheng X., Sun, D., Lu, F.*, Qin, H.-M.* Engineering of 3-ketosteroid-∆1-dehydrogenase based site-directed saturation mutagenesis for efficient biotransformation of steroidal substrates. Microb. Cell Fact. 2018, 17:141

3) Mao, S., Li, K., Hou, Y., Liu, Y., Ji, S., Qin, H.-M.*, Lu, F.* Synergistic effects of components in deep eutectic solvents relieve toxicity and improve the performance of steroid biotransformation catalyzed by Arthrobacter simplex, J. Chem. Technol. Biotechnol. 2018, 93: 2729–2736

4) Qin, H.-M., Xu, P., Guo, Q., Cheng, X., Gao, D., Sun, D., Zhu, Z., Lu, F., Biochemical characterization of a novel ulvan lyase from Pseudoalteromonas sp. strain PLSV. RSC Adv. 2018, 8: 2610-2615

5) Mao, S., Liu, Y., Hou, Y., Ma, X., Yang, J., Han, H., Wu, J., Jia, L., Qin, H.*, and Lu, F.* Efficient production of sugar-derived aldonic acids by Pseudomonas fragi TCCC11892, RSC Adv. 2018, 8: 39897-39901

6) Sun, D., Gao, D., Xu, P., Guo, Q., Zhu, Z., Cheng, X., Bai, S., Qin, H.-M.*, Lu, F.* A novel L-leucine 5-hydroxylase from Nostoc piscinale unravels unexpected sulfoxidation activity toward L-methionine, Protein Expres. Purif. 2018, 149, 1-6

获奖情况

2009-2013 日本政府（文部科学省）国费奖学金——大使馆推荐

2013年 东京大学农学生命科学研究科长奖励

联系方式

办公地点：天津经济技术开发区第十三大街29号天津科技大学生物工程学院

邮政编码：300457

办公电话：022-60602949

Email： huiminqin(*)tust.edu.cn, 为避免垃圾邮件将(*)更换为@