Rodrigo Pestana Lopes · Becton, Dickinson and Company接着解释道：

The basics of doublet discrimination is to detect disproportions between cell size vs. cell signal. Digital instruments have a function that allows one to correlate A and H so that they present the same variation when PMT voltages are changed, so that they can be correlated one to the other. In BD instruments we call this Area Scaling. When this factor is set properly, even though the voltage pulse is different between A and H, they will have the same reported value (that will be used to represent this particular event in the dot plot). So, if anyone has ever positioned the same parameter on both axis of a dot plot, you'll know that the graph that will apear is a perfect diagonal line (45 degrees, passing through ZERO). So, the basics of A vs. H strategy is, assuming that the instrument is set correctly and that A equals H, when correlating the same parameter (for instance, FSC) A vs. H, the same cell will have the same (or very similar) value in both axis. Therefore, all singlet events will be presented in a more diagonal display than doublets.