BFP :: Fluorescent Protein Database |
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BFP
a.k.a. P4-3 4 BFP is a basic (constitutively fluorescent) blue fluorescent protein published in 1996, derived from Aequorea victoria.No spectrum has been submitted ... add a spectrum! Oligomerization Organism Molecular Weight Cofactor Dimer Aequorea victoria 26.8 kDa - FPbase ID: SDS4K Attributes Ex λ Em λ EC (M-1 cm-1) QY Brightness pKa Maturation (min) Lifetime (ns) 381 445 14,000 0.38 5.32 Edit States/Attributes PhotostabilityNo photostability measurements available ... add one! BFP SequenceBFP was derived from P4 with the following mutations: Y145FY145F MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFSHGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYK ExcerptsNo excerpts have been added for BFPExcerpts are snippets from publications that capture key information about this protein that does not easily fit into one of the existing fields (such as a summary, motivation, or observation). Primary Reference Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transferHeim R, Tsien Ry (1996). Current Biology, 6(2) , 178-182. doi: 10.1016/s0960-9822(02)00450-5. Article Additional ReferencesNo additional references have been added. External Resources Search Addgene for BFP Change history Something missing or incorrect? Submit a change Submit a change |
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