Fabricating MnO2 Nanozymes as Intracellular Catalytic DNA Circuit Generators for Versatile Imaging of Base‐Excision Repair in Living Cells,Advanced Functional Materials |
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Fabricating MnO2 Nanozymes as Intracellular Catalytic DNA Circuit Generators for Versatile Imaging of Base‐Excision Repair in Living Cells,Advanced Functional Materials
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Nanomaterial/DNA integrated systems have become an emerging tool for intracellular imaging. However, intracellular catalytic DNA circuit is rarely explored. Commonly used nanosystems neglect intracellular DNA assembly, conformation folding and catalytic efficiency, all demanding appropriate metal ion conditions. Herein, MnO2 nanosheet/DNAzyme (nanozyme) is fabricated as intracellular catalytic DNA circuit generator for high signal amplification, and its operation is reported for monitoring DNA base‐excision repair (BER) in living cells with improved performance. MnO2 nanosheet works as not only DNA nanocarrier but also as DNAzyme cofactor supplier. The nanozyme is constructed by adsorbing DNA probes on MnO2 nanosheets, facilitating cellular uptake of DNA. They are rapidly released in cellular environments by reducing MnO2 nanosheets to Mn2+ as DNAzyme cofactor. After repair enzyme activation, nanozymes are properly assembled with active folded conformation and hold sustained catalytic efficiency over many cycles. It offers at least 40‐fold amplified signals for the monitoring of apurinic/apyrimidinic endonuclease‐initiated and DNA glycosylase‐initiated BER pathways. Multiplex imaging can be allowed by integrating several sets of probes with per MnO2 nanosheet. The MnO2 nanozyme opens up exciting opportunities for imaging low‐abundance biomarkers and relevant biological pathways in living cells.
中文翻译：
纳米材料/ DNA集成系统已成为细胞内成像的新兴工具。但是，很少探索细胞内催化DNA回路。常用的纳米系统会忽略细胞内DNA的组装，构象折叠和催化效率，所有这些都需要适当的金属离子条件。本文中，MnO 2纳米片/ DNAzyme（nanozyme）被制造为细胞内催化DNA电路发生器，用于高信号放大，据报道其操作可监测活细胞中DNA碱基切除修复（BER），从而提高性能。MnO 2纳米片不仅充当DNA纳米载体，而且还充当DNAzyme辅助因子的提供者。通过将DNA探针吸附在MnO 2上构建纳米酶纳米片，促进细胞对DNA的吸收。它们通过将MnO 2纳米片还原为Mn 2+作为DNAzyme辅因子而在细胞环境中迅速释放。修复酶活化后，纳米酶以有效的折叠构象正确组装，并在许多循环中保持持续的催化效率。它提供了至少40倍的放大信号，用于监测由嘌呤/嘧啶内切核酸酶引发的DNA糖基化酶引发的BER途径。通过将多组探针与每个MnO 2纳米片集成在一起，可以进行多重成像。MnO 2纳米酶为在活细胞中成像低丰度生物标志物和相关生物途径提供了令人兴奋的机会。 |
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