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载体基本信息
载体名称
pSoup
载体抗性
Tetracycline
载体长度
9275 bp
载体类型
Plant Vectors
载体来源
Hellens RP, Edwards EA, Leyland NR, Bean S, Mullineaux PM.
拷贝数
High copy number
3'引物
M13 rev
pSoup 载体载体图谱
pSoup9275 bp400800120016002000240028003200360040004400480052005600600064006800720076008000840088009200ColE1oriVmultiple cloning siteSP6 promoterM13 revlac operatorlac promoterCAP binding sitetet promotertetRTrfAreplicaseTetR
pSoup 载体载体序列
Copy Sequence
下载GeneBank文件(.gb)
LOCUS Exported 9275 bp ds-DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pSoup, complete sequence.
ACCESSION EU048870
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9275)
AUTHORS Hellens RP.
TITLE An update on the pGreen vector
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 9275)
AUTHORS Hellens RP.
TITLE Direct Submission
JOURNAL Submitted (21-JUL-2007) Horticulture and Food Research Institute of
New Zealand (HortResearch), 120 Mt. Albert Road, Auckland, NZ 1003,
New Zealand
REFERENCE 3 (bases 1 to 9275)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..9275
/organism="Cloning vector pSoup"
/mol_type="other DNA"
/db_xref="taxon:460120"
rep_origin 236..1094
/label=ColE1
/note="ColE1"
oriT 618..727
/note="incP origin of transfer"
CDS 760..1131
/codon_start=1
/gene="traJ"
/product="oriT-recognizing protein"
/label=traJ
/translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
KQDELGKVMMGVVRPRAEP"
rep_origin 1258..1969
/label=oriV
/note="incP origin of replication"
rep_origin 1276..1893
/label=OriV
/note="OriV"
misc_feature 2447..2498
/label=multiple cloning site
/note="multiple cloning site"
promoter complement(2505..2523)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(2541..2557)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 2565..2581
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(2589..2619)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2634..2655
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 2902..2930
/label=tet promoter
/note="E. coli promoter for tetracycline efflux protein
gene"
CDS 2978..4168
/codon_start=1
/gene="tetR"
/product="tetracyclin resistance protein"
/label=tetR
/protein_id="ABU39935.1"
/translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
gene 2978..4168
/gene="tetR"
/label=tetR
CDS 4667..5815
/codon_start=1
/gene="trfA"
/product="TrfA"
/label=trfA
/protein_id="ABU39936.1"
/translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
gene 4667..5815
/gene="trfA"
/label=trfA
CDS 7196..8167
/codon_start=1
/product="replicase"
/label=replicase
/note="pSa Rep; present on pGreen plasmid"
/protein_id="ABU39937.1"
/translation="MPKNNKAPGHRINEIIKTSLALEMEDAREAGLVGYMARCLVQATM
PHTDPKTSYFERTNGIVTLSIMGKPSIGLPYGSMPRTLLAWICTEAVRTKDPVLNLGRS
QSEFLQRLGMHTDGRYTATLRNQAQRLFSSMISLAGEQGNDFGIENVVIAKRAFLFWNP
KRPEDRALWDSTLTLTGDFFEEVTRSPVPIRIDYLHALRQSPLAMDIYTWLTYRVFLLR
AKGRPFVQIPWVALQAQFGSSYGSRARNSPELDDKARERAERAALASFKYNFKKRLREV
LIVYPEASDCIEDDGECLRIKSTRLHVTRAPGKGARIGPPPT"
CDS 8562..9212
/codon_start=1
/gene="tetR"
/product="tetracycline resistance regulatory protein"
/label=TetR
/translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
我们不提供该载体，载体信息仅供查询和参考。
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