Neural Cell Culture Protocols

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Neural Cell Culture Protocols

2024-07-15 11:40| 来源: 网络整理| 查看: 265

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Browse our Neurobiology protocols. You’ll find the protocols below along with the recommended components to build the optimal neural cell culture system for your research experiment.

Click on the protocol name to view the detailed protocol and full list of required materials.

Click on the product name in the right-hand columns to order.

细胞培养实验方案 Protocol Name 培养基 Supplement 细胞 Isolation, Culture, and Characterization of Cortical and Hippocampal Neurons

Neurobasal 培养基

Hibernate-E Medium

B-27 Serum Free Supplement Culturing Human Neural Stem CellsStemPro NSC SFM media kit (kit includes media, growth factors, and supplement)GlutaMAX™-I 补充剂Human neural stem cellsXeno-free Culture of Neural Stem CellsNeurobasal 培养基

B-27 Supplement, XenoFree

GlutaMAX™-I 补充剂

 Differentiating Neural Stem Cells into Neurons and Glial Cells

StemPro NSC SFM media kit (kit includes media, growth factors, and supplement)

Neurobasal 培养基

DMEM

B-27 Serum Free Supplement

N-2补充剂

GlutaMAX™-I 补充剂

Human neural stem cellsDifferentiating Glial Precursor Cells into Astrocytes and Oligodendrocytes

Neurobasal 培养基

Astrocyte Medium (kit includes medium and supplements)

B-27 Serum Free SupplementRat glial precursor cellsDerivation of Dopaminergic Neurons (from Human Embryonic Stem Cells)

DMEM

DMEM/F-12 with GlutaMax™ -I

Neurobasal 培养基

KnockOut™ 血清替代品(KSR)

N-2补充剂

B-27 Serum Free Supplement

Mouse embryonic fibroblastsCulturing Rat Fetal Neural Stem CellsStemPro NSC SFM media kit (kit includes media, growth factors, and supplement) Rat fetal neural stem cellsDerivation and Culture of Dopaminergic Neurons (from Midbrains of Rodents)Neurobasal 培养基B-27 Serum Free Supplement Derivation and Culture of Cortical AstrocytesAstrocyte Medium (kit includes medium and supplements)  Cryopreserving Neural Stem CellsStemPro NSC SFM media kit (kit includes media, growth factors, and supplement)  Cryopreservation and Recovery of Mature Differentiated Neural CellsNeurobasal 培养基B-27 Serum Free Supplement 

We understand that there’s more to your experiment than culturing your cells. View the protocols below for experiments beyond neural cell culture.

Click on the protocol name to view the detailed protocol and full list of required materials.

细胞分析

Protocol Name Cell Viability Assays for Neural Stem CellsMarkers for Characterizing Neural SubtypesSurface Marker Analysis by Flow CytometryImmunocytochemistryIntracellular Calcium Assay using Fluo-4

Molecular Characterization

Protocol Name PCR Primers for Molecular Characterization of Neural SubtypesRNA Isolation and cDNA Preparation from Neural Stem CellsCharacterizing Neural Cells by qPCR

转染

Protocol Name Transfecting Neural Cells Using the Neon Transfection SystemLipid-Mediated Transfection of Human Astrocytes

细胞培养资源 神经细胞培养产品选择3D 细胞培养出版物发行动态  

仅用于研究用途。 Not fur human or animal therapeutic or diagnostic use.



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