Molecular and genetic evidence for a tetrapolar mating system in Sparassis latifolia,Fungal Biology

您所在的位置:网站首页 mushroom中文翻译 Molecular and genetic evidence for a tetrapolar mating system in Sparassis latifolia,Fungal Biology

Molecular and genetic evidence for a tetrapolar mating system in Sparassis latifolia,Fungal Biology

2023-09-03 01:11| 来源: 网络整理| 查看: 265

Sparassis latifolia is a valuable edible fungus cultivated in East Asia that is rich in β-glucans. Understanding the mating system and sexual life cycle is important not only for breeding programs to improve strains but also for studies on speciation and population structures. In the present study, mating experiments using monokaryons derived from two different parental strains were performed. Chi-squared test indicated satisfied Mendel segregation, which supported a tetrapolar mating system. A search in the genome for homologs to the well-defined homeodomain and pheromone/receptors, as well as frequently found flanking genes, resulted in the identification of known mating-type loci previously identified in tetrapolar basidiomycetes, each represented by two idiomorphic alleles on separate contigs. Deficiency of the β-flanking protein in S. latifolia and S. crispa around the MAT-A locus may be explained by the locus being rich in transposable elements adjacent to HD genes. Monokaryotic mycelia are characterized by a slower growth rate and a relative lack of aerial mycelia compared with the parental strain. Chlamydospores can be produced in both monokaryotic and dikaryotic mycelial stages. We provide genetic and molecular evidence for the mating system of S. latifolia, a finding that will be helpful for the cross-breeding of this mushroom.

中文翻译:

Sparassis latifolia 四极交配系统的分子和遗传证据

Sparassis latifolia是东亚栽培的一种珍贵食用菌,富含β-葡聚糖。了解交配系统和性生命周期不仅对于育种计划以改善品系很重要,而且对于物种形成和种群结构的研究也很重要。在本研究中,进行了使用源自两种不同亲本菌株的单核细胞的交配实验。卡方检验表明满足孟德尔分离,支持四极交配系统。在基因组中搜索明确定义的同源域和信息素/受体的同源物,以及经常发现的侧翼基因,导致了先前在四极担子菌中鉴定的已知交配型基因座的鉴定,每个基因座由两个独立的自形等位基因代表重叠群。S. β-侧翼蛋白缺乏。MAT-A 基因座周围的阔叶和 S.crispa 可以通过该基因座富含与 HD 基因相邻的转座元件来解释。与亲本菌株相比,单核菌丝体的特点是生长速度较慢,气生菌丝体相对缺乏。厚壁孢子可以在单核和双核菌丝体阶段产生。我们为 S. latifolia 的交配系统提供了遗传和分子证据,这一发现将有助于这种蘑菇的杂交育种。



【本文地址】


今日新闻


推荐新闻


CopyRight 2018-2019 办公设备维修网 版权所有 豫ICP备15022753号-3