cDNA偼RNA傛傝偼暘夝偺怱攝偑彮側偄偺偱-20搙偱曐懚偟偰偄傞恖偑偨偄偰偄偩偲巚偄傑偡丅DNA偼EDTA側偳偑擖偭偨忬懺偱nuclease傪僽儘僢僋偟偰偍偔偲丄偁傞掱搙埲忋偺弮搙偱偁傟偽傑偢幒壏偱傕夡傟傞偙偲偼偁傝傑偣傫丅偨偩cDNA偼嶌偭偨梟塼偵Mg++側偳偑娷傑傟傞側偳丄枩偑堦nuclease偑偼偄傞偲暘夝偝傟傗偡偄娐嫬偱偼偁傝傑偡丅

偨偄偰偄4搙偱堦斢偖傜偄側傜cDNA偼戝忎晇偩傠偆偲偍傕偄傑偡偑丄偡傋偰偺働乕僗偱OK偲偄偆傎偳帺怣偼偁傝傑偣傫丅RT斀墳傪PCR儅僔乕儞偱偍偙側偭偰丄嵟屻4搙 for ever偺愝掕偱斀墳傪僗僞乕僩偝偣偰婣戭丄梻擔幚尡偲偄偆恖傕偄傞傫偠傖側偄偐側丅