发布者：Primerbank 时间：2017-12-12 浏览量：13113

Gene

Primer Sequence[5'-3']

Amplicon size(bp)

Intervening sequence size(bp)

Amplification efficiency(%)

ACTB

F: GGCACCCAGCACAATGAAG R: CCGATCCACACGGAGTACTTG

66

178

98

GAPDH

F: AGATCCCTCCAAAATCAAGTGG R: GGCAGAGATGATGACCCTTTT

130

220

98

GUSB

F: GAAAATATGTGGTTGGAGAGCTCATT R: CCGAGTGAAGATCCCCTTTTTA

101

3360

93

HMBS

F: CACGATCCCGAGACTCTGCT R: TACTGGCACACTGCAGCCTC

81

315

104

HPRT1

F: TGAGGATTTGGAAAGGGTGT R: GAGCACACAGAGGGCTACAA

118

1833

99

TBP

F: GAGCTGTGATGTGAAGTTTCC R: TCTGGGTTTGATCATTCTGTAG

117

1747

110

18S rRNA

F: GGAGTATGGTTGCAAAGCTGA R: ATCTGTCAATCCTGTCCGTGT

129

_

89

来源https://bmcmolbiol.biomedcentral.com/articles/10.1186/1471-2199-10-17