After isolation of peptides, salts and buffers can be removed using reversed phase (RP) resins, of which the C18 matrix is the most ideal for the capture of hydrophobic peptides. The peptides bind to reverse-phase columns in high-aqueous mobile phase, salts and buffers are washed off, and the peptides are eluted using a high-organic mobile phase. However, hydrophilic peptides, including phosphopeptides, may bind to C18 resins poorly, so graphite spin columns are recommended for better peptide recovery. However, C18 and graphite resins do not efficiently remove detergents, which can contaminate instruments and interfere with column binding, elution and ionization. The Thermo Scientific Detergent Removal products efficiently bind to and remove high concentrations of a wide variety of detergents that are commonly used during sample processing. Comprehensive—resins and kits for multiple peptide clean-up strategiesOptimized—all products are designed to maximize peptide yield and LC-MS compatibilityConvenient—easy-to-use spin tip and columns enable more rapid clean-up of peptidesFlexible—devices can be used with either cultured mammalian cells or tissuesValidated—all products have been fully tested and processed samples have been analyzed using Thermo Scientific Mass Spectrometers
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