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EcoRI has a High Fidelity version EcoRI-HF® (NEB #R3101).
High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 minutes with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.
For details on NEB’s quality controls for restriction endonucleases, visit our Restriction Enzyme Quality page.
Product Source
An E. coli strain that carries the cloned EcoRI gene from E. coli RY13 (R.N. Yoshimori).
This product is related to the following categories:
Restriction Endonucleases C G Products,
Time-Saver Qualified Restriction Enzymes Products
This product can be used in the following applications:
Fast Cloning: Accelerate your cloning workflows with reagents from NEB,
Restriction Enzyme Digestion
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