NLRP3重组抗体[EPR23094 |
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All lanes : Anti-NLRP3 antibody [EPR23094-1] (ab263899) at 1/500 dilution Lane 1 : Wild-type THP-1 cell lysateLane 2 : NLRP3 knockout THP-1 cell lysate Lysates/proteins at 20 µg per lane. Performed under reducing conditions. Predicted band size: 118 kDaObserved band size: 118 kDa False colour image of Western blot: Anti-NLRP3 antibody [EPR23094-1] staining at 1/500 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab263899 was shown to bind specifically to NLRP3. A band was observed at 118 kDa in wild-type THP-1 cell lysates with no signal observed at this size in NLRP3 knockout cell line ab280063 (knockout cell lysate ab280122). To generate this image, wild-type and NLRP3 knockout THP-1 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) at 1/20000 dilution. |
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