DHE (Dihydroethidium) Assay Kit - Reactive Oxygen Species (ab236206) measures ROS directly in live cells. This kit uses DHE as a fluorescent probe for the detection of ROS generation and is specific for superoxide and hydrogen peroxide. Antimycin A, an inhibitor of complex III of the mitochondrial electron transport chain, is included as a positive control for ROS generation. N-acetyl Cysteine is included as an anti-oxidant control.

ROS generation is represented as total DHE fluorescence. It is important that positive and negative controls be included in every experiment for all cell types tested. Data can be expressed as total fluorescence, geometric mean fluorescence (flow cytometry) or as % or controls.