mcherry激发波长是多少?

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mcherry激发波长是多少?

2024-07-09 18:15| 来源: 网络整理| 查看: 265

mcherry激发波长是多少?

作者:时间:2019-09-05 10:26浏览13263 次

信息摘要:

mCherry是一种来自于蘑菇珊瑚(mushroom coral)的红色荧光蛋白,常有于标记和示踪某些分子和细胞组分。相对于其他荧光,mCherry的好处在于它的颜色和应用最多的绿色荧光蛋白(GFP)能进行共同标记,并且mCherry相对于其他单体荧光蛋白来说也具有卓越的光稳定型。

mcherry激发波长是多少?

mCherry是一种来自于蘑菇珊瑚(mushroom coral)的红色荧光蛋白,常有于标记和示踪某些分子和细胞组分。相对于其他荧光,mcherry的好处在于它的颜色和应用最多的绿色荧光蛋白(GFP)能进行共同标记,并且mcherry相对于其他单体荧光蛋白来说也具有卓越的光稳定型。

吸收和发射波长:

mcherry的更大吸收/发射峰分别位于587nm和610nm,对光致漂白耐受,荧光非常稳定。

常见应用:•mCherry常用于与目的基因组成融合蛋白以及通过IRES或2A与感兴趣的蛋白共表达;•启动子活性研究;•荧光共振能量转移(fluorescence resonance energy transfer,FRET)和其他定量实验;•标记细胞或者分子,进行示踪实验;

 mCherry is a fluorophore (a fluorescent protein) used in biotechnology as a tracer to follow the flow of fluids, as a marker when tagged to molecules and cell components. mCherry and the majority of red fluorescent proteins derive from a protein isolated from Discosoma sp., while other fluorescent proteins in the green range are often variants of GFP from Aequorea victoria.

 m前缀的意义:mCherry的m为monmer单体的缩写,表示mCherry荧光蛋白的形式为单体,这在很多实验设计中非常重要,比如与目的标记基因组成融合蛋白时。

成熟时间:mCherry具有较快的成熟速度,t0.5为15分钟,这在一些需要做出快速反应的实验非常重要。比如启动子活性报告系统等。

如何观察和筛选mcherry在植物中的表达?

你可以选用LUYOR-3415RG和LUYOR-3260GR便携式荧光蛋白激发光源来直接观察和筛选mcherry在植物中有没有表达。如需进一步了解,请拨打电话153-1756-5658进行咨询(或直接添加微信15317565658咨询)。

mcherry红色荧光蛋白的激发波长和发射波长

上图为LUYOR-3415RG便携式红色荧光蛋白激发光源

上图为LUV-50A红色荧光蛋白观察眼镜

上图为LUV-590A 红色荧光蛋白拍照滤镜

上图为红色荧光蛋白在大豆根系上的表达(LUYOR-3415RG照射,LUV-590A滤镜拍摄)

实验室常见荧光蛋白的激发波长和发射波长

紫外荧光蛋白UV Proteins

Protein Excitation Wavelength   Emission Wavelength        Sirius 355 424 Sandercyanin 375 630 shBFP-N158S/L173I 375 458

蓝色荧光蛋白Blue Proteins

Protein Excitation Wavelength Emission Wavelength Azurite 383 450 EBFP2 383 448 mKalama1 385 456 mTagBFP2                        399 454 TagBFP 402 457 shBFP 401 458

青色荧光蛋白Cyan Proteins

Protein Excitation Wavelength Emission Wavelength ECFP 433 475 Cerulean 433 475 mCerulean3 433 475 SCFP3A 433 474 CyPet 435 477 mTurquoise 434 474 mTurquoise2 434 474 TagCFP 458 480 mTFP1 462 492 monomeric Midoriishi-Cyan 470 496 Aquamarine 430 474

绿色荧光蛋白Green Proteins

Protein Excitation Wavelength Emission Wavelength TurboGFP 482 502 TagGFP2 483 506 mUKG 483 499 Superfolder GFP 485 510 Emerald 487 509 EGFP 488 507 Monomeric Azami Green    492 505 mWasabi 493 509 Clover 505 515 mNeonGreen 506 517 NowGFP 494 502 mClover3 506 518

黄色荧光蛋白Yellow Proteins

Protein Excitation Wavelength Emission Wavelength TagYFP 508 524 EYFP 513 527 Topaz 514 527 Venus 515 528 SYFP2 515 527 Citrine 516 529 Ypet 517 530 lanRFP-ΔS83                       521 592 mPapaya1 530 541 mCyRFP1 528 594

桔色荧光蛋白Orange Proteins

Protein Excitation Wavelength Emission Wavelength Monomeric Kusabira-Orange 548 559 mOrange 548 562 mOrange2 549 565 mKOκ 551 563 mKO2 551 565

红色荧光蛋白Red Proteins

Protein Excitation Wavelength Emission Wavelength TagRFP 555 584 TagRFP-T 555 584 RRvT 556 583 mRuby 558 605 mRuby2 559 600 mTangerine                             568 585 mApple 568 592 mStrawberry 574 596 FusionRed 580 608 mCherry 587 610 mNectarine 558 578 mRuby3 558 592 mScarlet 569 594 mScarlet-I 569 593

远红荧光蛋白Far Red Proteins

Protein Excitation Wavelength Emission Wavelength mKate2 588 633 HcRed-Tandem 590 637 mPlum 590 649 mRaspberry 598 625 mNeptune 600 650 NirFP 605 670 TagRFP657                             611 657 TagRFP675 598 675 mCardinal 604 659 mStable 597 633 mMaroon1 609 657 mGarnet2 598 671

近红荧光蛋白Near IR Proteins

Protein Excitation Wavelength Emission Wavelength iFP1.4 684 708 iRFP713 (iRFP)                      690 713 iRFP670 643 670 iRFP682 663 682 iRFP702 673 702 iRFP720 702 720 iFP2.0 690 711 mIFP 683 704 TDsmURFP 642 670 miRFP670 642 670

Sapphire-type Proteins

Protein Excitation Wavelength Emission Wavelength Sapphire 399 511 T-Sapphire                              399 511 mAmetrine 406

526

Red Fluorescent Proteins

Red fluorescent proteins (RFP) can be imaged on existing confocal or widefield microscopes, and they also have more penetrating power. The excitation and emission maxima of RFP are 558nm and 583 nm, respectively.

The use of RFP, however, has been hampered with several issues. RFP is an obligate tetramer - thus, it forms large aggregates inside cells. This makes the use to RFP to report the location of a protein severely limited.

Although GFP can successfully fuse with several hundreds of proteins, RFP-conjugated proteins are often toxic. Some variants of RFP have overcome these limitations. For example, DsRed2 fluorescent protein does not form aggregates and has reduced toxicity, while another variant of RFP (known as RedStar) has increased brightness and maturation rate.

未经公司同意,严禁转载我站文章,经同意转载的,必须注明出处。 【相关文章】 如何选择荧光蛋白的激发波长? LUYOR-3430RB用于检测钙黄绿素在鱼的表达 转基因植物的GFP发光检测能用什么灯直接观察么? mcherry激发波长是多少? 【相关产品】 LUYOR-3415RG双波长便携式荧光蛋白激发光源 LUYOR-3410高强度紫外线灯 LUYOR-3260荧光蛋白激发光源 LUYOR-3430体视显微镜荧光激发光源 上一条 查看详情 +转基因植物的GFP发光检测能用什么灯直接观察么? 下一条 查看详情 + 为什么在紫外光照射下绿色荧光蛋白能发光? 返回列表 本文标签: 绿色荧光蛋白 gfp 荧光蛋白 荧光蛋白激发波长 荧光蛋白激发光源 红色荧光蛋白


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