1. 在Matlab命令窗口输入命令：

help imwrite

会有如下解释：

If the input array is of class uint16 and the format supports 16-bit data (JPEG, PNG, and TIFF), imwrite outputs the data as 16-bit values. If the format does not support 16-bit values, imwrite issues an error. Several formats, such as JPEG and PNG, support a parameter that lets you specify the bitdepth of the output data.

If the input array is of class double, and the image is a grayscale or RGB color image, imwrite assumes the dynamic range is [0,1] and automatically scales the data by 255 before writing it to the file as 8-bit values.

2. 如果你要保存成uint16的灰度图像的矩阵数据是非uint16的类型：

则必须先将double类型转换为uint16的类型，执行命令：

结果如下：

3. 然后执行命令：

imwrite(data11,'image.png','png','bitdepth',16);

最终得到uint16的灰度图像image.png。如下图所示：

参考网址：https://wenda.so.com/q/1534806305217644